Hydrogen sulfide is a toxic gas involved in the regulation of some essential biological processes. A novel, precise, accurate and rapid method based on high-performance liquid chromatography with diode array detection for the determination of sulfide ions in human urine sample is proposed. The method involves the derivatization of sulfide with pyrylium salts – (2,4,6-triphenylpyrylium hydrogensulfate(VI) (L1) and 4-[p-(N,N-dimethylamino)phenyl]-2,6-diphenylpyrylium chlorate(VII) (LN1). The separation occurs on InfinityLab Poroshell 120 EC C₁₈ column using acetonitrile and phosphate buffer as a mobile phase. The detectors utilized a wavelength of 371 or 580 nm. The calibration curves were linear in the range of 2–150 μmol L⁻¹ and 1–50 μmol L⁻¹ for L1 and LN1 derivatives, respectively. The samples were found to be stable from sample collection to final analysis. The method was successfully applied to samples from apparently healthy volunteers.

硫化氢是有毒气体，参与某些必不可少的生物过程的调节。提出了一种基于高效液相色谱-二极管阵列检测的新颖，准确，准确，快速的方法，用于测定人尿中的硫化物。该方法涉及用吡啶鎓盐–（2,4,6-三苯基吡啶鎓硫酸氢盐（VI）（L1）和4- [对-（N，N-二甲基氨基）苯基] -2,6-二苯基吡啶鎓氯酸盐（VII）衍生化硫化物。 ）（LN1）。分离在乙腈和磷酸盐缓冲液作为流动相的InfinityLab Poroshell 120 EC C ₁₈色谱柱上进行的，检测器使用的波长为371或580 nm，校正曲线在2–150μmol范围内呈线性L ^-1和1–50μmolL ^-1分别用于L1和LN1导数。发现样品从样品收集到最终分析都是稳定的。该方法已成功应用于来自显然健康的志愿者的样品。