In the efforts for the removal of hazardous materials from the environment biological processes are a valuable tool. Although much attention has been paid to the changes in bacteria at the omics level, another, physical-chemical perspective on the issue is essential, as little is known of microbial response to continuous exposition on harmful substances. This study provides in-depth characterization of the physical-chemical parameters of bacterial biomass after hydrocarbons exposure. To provide comparability of the harmful effects of chlorotoluenes and xylenes non-exposed and 12-months hydrocarbons exposed cells were analyzed, using the advanced spectrometric methods, inverse gas chromatography and low-temperature N2 sorption to evaluate acid-base as well as dispersive properties of the studied biomass. Presented results indicate P. fluorescens B01 cells strategy aimed at protecting the cell, thus lowering its’ biodegradation efficiency as a result of metabolic stress. The outcome of the study was that prolonged exposure to pollutants might reduce the bioavailability of hydrocarbons to bacteria cells, and consequently decrease the effectiveness of decontamination of polluted sites by indigenous microorganisms.

在努力从环境中去除有害物质的过程中，生物过程是一种有价值的工具。尽管在组学水平上已经对细菌的变化给予了很多关注，但是，关于该问题的另一种物理化学观点是必不可少的，因为人们对微生物对有害物质连续暴露的反应知之甚少。这项研究提供了碳氢化合物暴露后细菌生物量的物理化学参数的深入表征。为了提供未暴露的氯甲苯和二甲苯和暴露于12个月的碳氢化合物的有害影响的可比性，使用了先进的光谱分析方法，反相气相色谱法和低温N2吸附来评估酸碱和分散性，研究的生物量。显示结果表明荧光假单胞菌B01细胞策略旨在保护细胞，从而由于代谢应激而降低其生物降解效率。该研究的结果是，长时间暴露于污染物中可能会降低碳氢化合物对细菌细胞的生物利用度，从而降低本地微生物对污染部位进行净化的效率。