Biomimetic hydrogels which possess good biocompatibility, high degradability, and low toxicity as well as good antibacterial activity against various bacteria would potentially be promising for biomaterial applications, such as wound healing, tissue engineering, and payload delivery systems. Herein, we report the synthesis and hydrogelation of L-Dopa conjugated (GPLD) polypeptides via a versatile strategy including enzymatic cross-linking or coordinated/oxidized cross-linking with Fe³⁺ ions and demonstrated the feasibility of loading cancer drug and metal NPs in hydrogel matrix. The drug-loaded hydrogel was simply prepared via coordinated/oxidized cross-linking by Fe³⁺ and H₂O₂ within short gelation time. Doxorubicin (DOX) was encapsulated in the hydrogel network through the formation of metal-DOX/catechol complexes. The catechol groups acted not only as the complexing depots for DOX encapsulation but also as cross-linking depots for hydrogel formation. The mechanical strength, swelling ratio, and degradability behavior could be tuned by varying Fe³⁺/H₂O₂ or enzyme/H₂O₂ concentration. The as-prepared hydrogel exhibited excellent pH-responsive drug release behavior and the ability to effectively kill cancer cells by pH-triggered release of DOX. We also demonstrated that the enzymatically cross-linked hydrogels loaded with metal nanoparticles (NPs) exhibiting excellent antimicrobial activities. This multifunctional hydrogel is promising for drug delivery and antimicrobial applications.

具有良好的生物相容性，高降解性和低毒性以及对各种细菌的良好抗菌活性的仿生水凝胶将有望用于生物材料应用，例如伤口愈合，组织工程和有效载荷输送系统。在这里，我们报告的L-多巴共轭（GPLD）多肽的合成和水凝胶化通过一种通用的策略，包括酶促交联或与Fe ³⁺离子的配位/氧化交联，并证明了装载抗癌药物和金属NPs的可行性水凝胶基质。通过Fe ³⁺和H ₂ O _2的配位/氧化交联，可以简单地制备载药水凝胶在较短的胶凝时间内。阿霉素（DOX）通过形成金属-DOX /邻苯二酚复合物而被包封在水凝胶网络中。邻苯二酚基团不仅充当DOX封装的络合库，而且还充当水凝胶形成的交联库。可以通过改变Fe ³⁺ / H ₂ O ₂或酶/ H ₂ O ₂来调节机械强度，溶胀率和降解性能。浓度。所制备的水凝胶表现出优异的pH响应药物释放行为，并具有通过pH触发的DOX释放有效杀死癌细胞的能力。我们还证明了负载金属纳米颗粒（NPs）的酶促交联水凝胶表现出优异的抗菌活性。这种多功能水凝胶有望用于药物输送和抗菌应用。