The extended use of niosomes in food, cosmetic and pharmaceutical industries demands novel preparation processes which could enhance nanovesicles production with controlled size and size distribution. Microfluidics (MF) are a novel promising set of preparation methods for this purpose. Studies comparing methods for niosome preparation are still scarce.

In this work, niosomes using MF were produced and the effect of membrane compounds concentration and the relation between organic and aqueous ratio (flow rate ratio, FRR) on the resulting mean size and size distribution were studied. Two different cholesterol free formulations were tested using Tween® 80 and Span® 80 in presence and absence of 1-dodecanol as membrane stabilizer. The effect of FRR showed to be the key parameter in niosomes production, being more critical than membrane compounds concentration and the vesicle membrane composition.

Additionally, the same formulations were also produced by two conventional methods, thin film hydration (TFH) and ethanol injection (EI), and results were compared. The size of the niosomes obtained by MF was smaller and more homogeneous than those achieved by TFH and similar to EI. Therefore, MF is confirmed as a novel technology that can facilitate the development and optimization of new nanodelivery systems with controlled size and polydispersity, two essential parameters for successful biotechnological applications.

脂质体在食品，化妆品和制药工业中的广泛使用要求新颖的制备方法，该方法可以在控制尺寸和尺寸分布的情况下提高纳米囊泡的生产。微流体（MF）是为此目的而提出的一组新颖的制备方法。比较用于制备脂质体的方法的研究仍然很少。

在这项工作中，制备了使用MF的脂质体，并研究了膜化合物浓度以及有机物和水的比率（流速比，FRR）之间的关系对所得平均粒径和粒径分布的影响。在存在和不存在1-十二烷醇作为膜稳定剂的情况下，使用Tween®80和Span®80测试了两种不同的不含胆固醇的制剂。FRR的影响被证明是生产脂质体的关键参数，比膜化合物的浓度和囊泡膜的组成更为关键。

此外，还通过两种常规方法（薄膜水化（TFH）和乙醇注射（EI））生产了相同的制剂，并比较了结果。MF制得的脂质体的大小比TFH制得的更小，更均匀，与EI相似。因此，MF被确认为一项新颖的技术，可以促进具有受控的尺寸和多分散性（成功的生物技术应用的两个基本参数）的新型纳米递送系统的开发和优化。