Background

Human endogenous retroviruses (HERVs) constitute around 8% of the human genome and have important roles in human health and disease, including cancers. Previous studies showed that HERV envelope (Env) proteins are highly expressed in cancer tissues and co-related with cancer progression. KAP1 has been reported to play a key role in regulating retrotransposons, including HERV-K, through epigenetic silencing.

Objective

The relationship between KAP-1 and HERV Envs expressions was analyzed only in tumor cell lines and has not yet been studied in cancer tissues. In this study, we analyzed the expression patterns and relationship between KAP1 and HERV Env proteins in ovarian cancer tissues.

Method

The expression patterns of KAP-1 and HERV Env proteins, including HERV-K and HERV-R, were analyzed in ovarian cancer tissue microarrays that contained 80 surgical specimens, including normal ovary and malignant ovarian cancers.

Results

The expression of HERV-R Env and KAP1 proteins is significantly higher in ovarian cancer compared with normal ovary tissues. However, the expression of HERV-K Env did not change significantly in cancer tissues. The expression patterns of HERV-K Env and HERV-R Env significantly increased in early stages of cancer and KAP1 expression was higher in certain stage and types of cancers. However, the expression of HERV-K Env, HERV-R Env, and KAP1 did not change in different age groups. The correlation between the expression of KAP1 and HERV-Env, including HERV-K and HERV-R, was not significantly correlated.

Conclusions

The results of this study showed that there was no significant correlation between the expression of KAP1 and HERV Env proteins in ovarian cancer tissues, unlike studies with cell lines in vitro. These results suggest that the actual expression of HERV Env proteins in ovarian cancer tissues may be regulated through various complex factors as well as KAP1.

中文翻译：

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卵巢癌中 KAP1 表达模式和人内源性逆转录病毒 Env 蛋白的分析。

背景

人类内源性逆转录病毒 (HERV) 约占人类基因组的 8%，在人类健康和疾病（包括癌症）中具有重要作用。先前的研究表明，HERV 包膜 (Env) 蛋白在癌组织中高度表达并与癌症进展相关。据报道，KAP1 通过表观遗传沉默在调节逆转录转座子（包括 HERV-K）中发挥关键作用。

客观的

KAP-1 和 HERV Envs 表达之间的关系仅在肿瘤细胞系中进行了分析，尚未在癌组织中进行研究。在本研究中，我们分析了卵巢癌组织中 KAP1 和 HERV Env 蛋白的表达模式和关系。

方法

在包含 80 个手术标本（包括正常卵巢癌和恶性卵巢癌）的卵巢癌组织微阵列中分析了 KAP-1 和 HERV Env 蛋白（包括 HERV-K 和 HERV-R）的表达模式。

结果

HERV-R Env 和 KAP1 蛋白在卵巢癌中的表达明显高于正常卵巢组织。然而，HERV-K Env的表达在癌组织中没有显着变化。HERV-K Env 和 HERV-R Env 的表达模式在癌症的早期阶段显着增加，并且 KAP1 表达在某些阶段和类型的癌症中更高。然而，HERV-K Env、HERV-R Env和KAP1的表达在不同年龄段没有变化。KAP1 和 HERV-Env，包括 HERV-K 和 HERV-R 的表达之间没有显着相关性。

结论

本研究的结果表明，与体外细胞系研究不同，卵巢癌组织中 KAP1 和 HERV Env 蛋白的表达之间没有显着相关性。这些结果表明，卵巢癌组织中HERV Env蛋白的实际表达可能受多种复杂因素以及KAP1的调控。