Photo-responsive photonic hydrogel: in situ manipulation and monitoring of cell scaffold stiffness,Materials Horizons

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Photo-responsive photonic hydrogel: in situ manipulation and monitoring of cell scaffold stiffness
Materials Horizons ( IF 12.2 ) Pub Date : 2020-08-11 , DOI: 10.1039/d0mh01019g
Sen Li _{1,

2,

3,

4,

5} , Yi Zeng _{1,

2,

3,

4,

5} , Wei Hou _{1,

2,

3,

4,

5} , Wang Wan _{1,

2,

3,

4,

5} , Junning Zhang _{1,

2,

3,

4,

5} , Yuli Wang _{3,

4,

6,

7,

8} , Xin Du _{1,

2,

3,

4,

5} , Zhongze Gu _{1,

2,

3,

4,

5}

Affiliation

Building scaffolds with heterogeneous and reconfigurable mechanical properties has been recognized as the key to recapitulating the natural extracellular microenvironments for in vitro cell incubation systems. However, in situ changing and tracking of the mechanical change of cell scaffolds are still challenging. In this paper we developed a novel strategy to solve this problem. We show a convenient method for tracking the hardness of cell scaffolds in a non-invasive manner via their color change. This is achieved by introducing colloidal crystal features into a photo-reconfigurable hydrogel, which leads to a smart hydrogel scaffold with dynamically adjustable and in situ monitorable mechanical properties. The stiffness of the hydrogel can be spatiotemporally and dynamically manipulated by light; meanwhile, its changes can be in situ recognized by analyzing the hydrogel color. This allows real-time control and investigation of the extracellular stiffness in cell incubation systems in a non-invasive fashion. In addition, the cell–matrix interactions on this smart scaffold can be easily understood owing to the simultaneous observation of cell morphology and matrix stiffness under a microscope. We also show that such a hydrogel could be used as a user-designable microarray for cell culture applications.

中文翻译：

光响应性光子水凝胶：原位操作和细胞骨架硬度的监测

具有异质且可重新配置的机械性能的建筑支架已被公认为为体外细胞培养系统概括天然细胞外微环境的关键。然而，原位改变和跟踪细胞支架的机械改变仍然具有挑战性。在本文中，我们开发了一种新颖的策略来解决此问题。我们展示了一种方便的方法来通过其颜色变化以非侵入性的方式跟踪细胞支架的硬度。这是通过将胶体晶体特征引入可光重构的水凝胶中来实现的，从而形成了具有动态可调和原位功能的智能水凝胶支架可监控的机械性能。水凝胶的刚度可以通过光在时空上动态地控制。同时，通过分析水凝胶的颜色可以原位识别其变化。这允许以无创方式实时控制和研究细胞培养系统中的细胞外硬度。此外，由于在显微镜下同时观察细胞形态和基质刚度，因此可以轻松理解该智能支架上的细胞-基质相互作用。我们还表明，这种水凝胶可用作细胞培养应用的用户可设计的微阵列。

更新日期：2020-09-01

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