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Acoustofluidic multi-well plates for enrichment of micro/nano particles and cells.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-08-11 , DOI: 10.1039/d0lc00378f Pengzhan Liu 1 , Zhenhua Tian , Nanjing Hao , Hunter Bachman , Peiran Zhang , Junhui Hu , Tony Jun Huang
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-08-11 , DOI: 10.1039/d0lc00378f Pengzhan Liu 1 , Zhenhua Tian , Nanjing Hao , Hunter Bachman , Peiran Zhang , Junhui Hu , Tony Jun Huang
Affiliation
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Controllable enrichment of micro/nanoscale objects plays a significant role in many biomedical and biochemical applications, such as increasing the detection sensitivity of assays, or improving the structures of bio-engineered tissues. However, few techniques can perform concentrations of micro/nano objects in multi-well plates, a very common laboratory vessel. In this work, we develop an acoustofluidic multi-well plate, which adopts an array of simple, low-cost and commercially available ring-shaped piezoelectric transducers for rapid and robust enrichment of micro/nanoscale particles/cells in each well of the plate. The enrichment mechanism is validated and characterized through both numerical simulations and experiments. We observe that the ring-shaped piezoelectric transducer can generate circular standing flexural waves in the substrate of each well, and that the vibrations can induce acoustic streaming near the interface between the substrate and a fluid droplet placed within the well; this streaming can drive micro/nanoscale objects to the center of the droplet for enrichment. Moreover, the acoustofluidic multi-well plate can realize simultaneous and consistent enrichment of biological cells in each well of the plate. With merits such as simplicity, controllability, low cost, and excellent compatibility with other downstream analysis tools, the developed acoustofluidic multi-well plate could be a versatile tool for many applications such as micro/nano fabrication, self-assembly, biomedical/biochemical sensing, and tissue engineering.
中文翻译：
用于富集微/纳米颗粒和细胞的声流控多孔板。
微米/纳米尺度物体的可控富集在许多生物医学和生化应用中发挥着重要作用，例如提高分析的检测灵敏度，或改善生物工程组织的结构。然而，很少有技术可以在多孔板（一种非常常见的实验室容器）中进行微米/纳米物体的浓缩。在这项工作中，我们开发了一种声流控多孔板，它采用一系列简单、低成本和市售的环形压电换能器，可以快速、稳健地富集板的每个孔中的微/纳米级颗粒/细胞。通过数值模拟和实验对富集机制进行了验证和表征。我们观察到环形压电换能器可以在每个孔的基底中产生圆形驻弯曲波，并且振动可以在基底和放置在孔内的流体滴之间的界面附近引起声流；这种流动可以将微米/纳米级物体驱动到液滴中心以进行富集。而且，声流控多孔板可以实现板内各孔生物细胞的同步、一致富集。所开发的声流控多孔板具有简单、可控、低成本以及与其他下游分析工具良好的兼容性等优点，可以成为微/纳米制造、自组装、生物医学/生化传感等许多应用的多功能工具和组织工程。
更新日期：2020-09-15
中文翻译：
用于富集微/纳米颗粒和细胞的声流控多孔板。
微米/纳米尺度物体的可控富集在许多生物医学和生化应用中发挥着重要作用，例如提高分析的检测灵敏度，或改善生物工程组织的结构。然而，很少有技术可以在多孔板（一种非常常见的实验室容器）中进行微米/纳米物体的浓缩。在这项工作中，我们开发了一种声流控多孔板，它采用一系列简单、低成本和市售的环形压电换能器，可以快速、稳健地富集板的每个孔中的微/纳米级颗粒/细胞。通过数值模拟和实验对富集机制进行了验证和表征。我们观察到环形压电换能器可以在每个孔的基底中产生圆形驻弯曲波，并且振动可以在基底和放置在孔内的流体滴之间的界面附近引起声流；这种流动可以将微米/纳米级物体驱动到液滴中心以进行富集。而且，声流控多孔板可以实现板内各孔生物细胞的同步、一致富集。所开发的声流控多孔板具有简单、可控、低成本以及与其他下游分析工具良好的兼容性等优点，可以成为微/纳米制造、自组装、生物医学/生化传感等许多应用的多功能工具和组织工程。
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