The effects of acrylamide on CO2 production from [14C]-labelled glucose, pyruvate and glutamine have been studied in a mouse neuroblastoma cell line C1300, clone N1E115. A rotation metabolism chamber, permitting closed incubation of monolayer, anchorage-dependent cell cultures under good physiological conditions, was developed for making the determinations. The cells were exposed to acrylamide (0.35mM) for 14 days. The total amunt of CO2 produced from glucose and pyruvate was increased by exposure to acrylamide, whereas a slight inhibition was found in the production from glutamine. The production of lactate remained unchanged. Comparison of these results with other data obtained in our laboratory leads us to conclude that the method described is relevant for the determination of energy metabolic processes through the quantification of CO2 production. Furthermore, we assume that acrylamide causes an increased demand for energy in the cells and that this demand is met by the cells through the increased oxidative phosphorylation of glucose.

中文翻译：

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基于滚轮室技术测定培养细胞中 CO2 产生的方法：丙烯酰胺对神经母细胞瘤 N1E115 培养物的影响

已经在小鼠神经母细胞瘤细胞系 C1300 克隆 N1E115 中研究了丙烯酰胺对 [14C] 标记的葡萄糖、丙酮酸和谷氨酰胺产生 CO2 的影响。开发了一个旋转代谢室，允许在良好的生理条件下封闭培养单层贴壁依赖性细胞培养物，以进行测定。细胞暴露于丙烯酰胺 (0.35mM) 14 天。暴露于丙烯酰胺后，葡萄糖和丙酮酸产生的 CO2 总量增加，而谷氨酰胺的产生受到轻微抑制。乳酸的产量保持不变。将这些结果与我们实验室获得的其他数据进行比较，我们得出结论，所描述的方法与通过量化 CO2 产生来确定能量代谢过程有关。此外，我们假设丙烯酰胺导致细胞对能量的需求增加，并且细胞通过增加葡萄糖的氧化磷酸化来满足这种需求。