The aim of the present study was to investigate the suitability of bovine spermatozoa cryopreserved in a “defined” medium as an in vitro model for the assessment of the cytotoxic potential of chemicals. The endpoints used for this purpose were motion activity and cellular ATP content. The evaluation of properties of cryopreserved sperm shortly after thawing and at the end of a one-hour incubation period, shows that the cryoprotective medium developed is able to provide suitable cellular material for cytotoxicity tests. Results from experiments employing substances with known modes of action are presented, and suggest that cryopreserved sperm can be used as successfully as native sperm in cytotoxicity tests.

中文翻译：

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用冷冻保存的牛精子进行毒性测试

本研究的目的是调查在“确定的”培养基中冷冻保存的牛精子作为评估化学品细胞毒性潜力的体外模型的适用性。用于此目的的终点是运动活动和细胞 ATP 含量。在解冻后不久和一小时孵育期结束时对冷冻保存精子的特性进行评估，表明开发的冷冻保护培养基能够为细胞毒性测试提供合适的细胞材料。介绍了使用具有已知作用方式的物质的实验结果，并表明冷冻保存的精子可以像天然精子一样成功地用于细胞毒性测试。