The spindle-inhibiting and aneuploidy-inducing potency of in vitro exposure of PHA-stimulated human lymphocytes to organotin compounds has been studied indirectly by quantitation of chromosomal contraction and directly by determination of the frequency of aneuploidy by chromosome counting. The effects of trimethyltin chloride (TMT), dimethyltin chloride (DMT), tributyltin chloride (TBT), dibutyltin chloride (DBT), triphenyltin chloride (TPhT), and diphenyltin chloride (DPhT) on chromosal contraction were studied at concentrations of 10-3-10 9M, by measurement of the average length of chromosome 1 from asynchronous cultures of human peripheral lymphocytes. TMT, TBT, TPhT and DPhT appear to be very strong inducers of chromosomal supercontraction, indicating that these compounds are possible spindle inhibitors, while DMT and DBT seem to be ineffective. TMT, TBT, TPhT and DPhT gave rise to a little more than 100% increase in the number of hyperdiploid cells, all the increases being statistically significant except for that induced by TBT, indicating that organotin compounds are capable of inducing aneuploidy, probably by affecting spindle function.

中文翻译：

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有机锡化合物非整倍体诱导效力的直接和间接评估

PHA 刺激的人淋巴细胞体外暴露于有机锡化合物的纺锤体抑制和非整倍体诱导效力已通过染色体收缩的定量和通过染色体计数直接测定非整倍体的频率进行了间接研究。研究了三甲基氯化锡 (TMT)、二甲基氯化锡 (DMT)、三丁基氯化锡 (TBT)、二丁基氯化锡 (DBT)、三苯基氯化锡 (TPhT) 和二苯基氯化锡 (DPhT) 在 10-3 浓度下对染色体收缩的影响-10 9M，通过测量来自人类外周淋巴细胞异步培养的 1 号染色体的平均长度。TMT、TBT、TPhT 和 DPhT 似乎是非常强的染色体超收缩诱导剂，表明这些化合物可能是纺锤体抑制剂，而 DMT 和 DBT 似乎无效。TMT、TBT、TPhT 和 DPhT 使超二倍体细胞的数量增加了 100% 以上，除 TBT 诱导的增加外，所有增加都具有统计学意义，表明有机锡化合物能够诱导非整倍体，可能是通过影响主轴功能。