Isoniazid, the choice antitubercular agent, has only been employed against Mycobacterium tuberculosis. This study evaluated if the enzyme-mimetic activities of magnetic nanoparticles could accelerate the activation process of isoniazid against mycobacterial and, more importantly, non-mycobacterial microorganisms. First, magnetic nanoparticles were synthesized and coated by lipoamino acid; then, isoniazid was conjugated to synthesized nanoparticles. Antibacterial activities of nanoconjugated isoniazid were evaluated against Mycobacterium tuberculosis and four Gram-positive and Gram-negative nonmycobacterial strains through a microdilution broth process. Results showed that the required amount of isoniazid against Mycobacterium tuberculosis would decrease to 44.8% and 16.7% in conjugation with naked and surface-modified magnetic nanoparticles, respectively. Also, 32 μg/mL and 38 μg/mL of isoniazid in conjugation with naked and surface-modified nanoparticles, respectively, could prevent the growth of Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Hence, the vicinity of magnetic nanoparticles with isoniazid could declare promising aspects of isoniazid antibacterial capabilities.

中文翻译：

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表面修饰的磁性纳米粒子与异烟肼共轭对结核分枝杆菌和非分枝杆菌微生物的抗菌活性

异烟肼是一种抗结核药物，仅用于抗结核分枝杆菌。这项研究评估了磁性纳米粒子的酶模拟活性是否可以加快异烟肼对分枝杆菌，更重要的是对非分枝杆菌微生物的激活过程。首先，合成磁性纳米颗粒并用脂氨基酸包被；然后，将异烟肼偶联到合成的纳米颗粒上。通过微量稀释肉汤法评估了纳米共轭异烟肼对结核分枝杆菌和四种革兰氏阳性和革兰氏阴性非分枝杆菌菌株的抗菌活性。结果表明异烟肼对结核分枝杆菌的需求量与裸露的和经表面改性的磁性纳米粒子的结合将分别降低至44.8％和16.7％。此外，32  μ克/毫升和38  μ与裸和表面改性的纳米粒子结合异烟肼克/毫升，分别可以防止生长粪肠球菌，大肠杆菌，铜绿假单胞菌，和金黄色葡萄球菌。因此，具有异烟肼的磁性纳米粒子附近可能表明异烟肼的抗菌能力很有希望。