General conditions and requirements for an internal standard useful in the determination of chemical warfare agents (CWAs) by the method of gas chromatography coupled with mass detection (GC/MS) were defined. The determination is based on a GC/MS analysis of a mixture of a CWA with an internal standard, conversion of the TIC chromatogram to a chromatogram extracted at a particular m/z ratio, and calculation of the CWA concentration from the internal standard concentration, response factor, and chromatographic peak areas. Available internal standards were identified, and they were verified for seven organophosphorus nerve-paralysing agents. Corresponding response factors were determined as a ratio of gradients of the linear functions of the peak area and compound concentration. Linearity, repeatability, and accuracy of the measurements were evaluated. The determination can be performed on all GC/MS systems of the Fire Rescue Service of the Czech Republic (FRS), where no CWA standards are available.

中文翻译：

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通过GC / MS方法对化学战剂进行定量分析的内部标准：神经毒剂。

定义了通过气相色谱与质量检测（GC / MS）的方法测定化学战剂（CWA）的内标的一般条件和要求。该测定基于CWA与内标混合物的GC / MS分析，将TIC色谱图转换为以特定m / z提取的色谱图比率，并根据内标浓度，响应因子和色谱峰面积计算CWA浓度。确定了可用的内标，并对其中的七种有机磷神经麻痹剂进行了验证。将相应的响应因子确定为峰面积和化合物浓度的线性函数的梯度之比。评估了线性，重复性和测量精度。可以在捷克共和国消防局（FRS）的所有GC / MS系统上执行此确定，因为该系统没有CWA标准。