The supplementation of ruminant diets with exogenous cellulolytic enzymes can improve their digestibility and feeding value. The objective of this study was to determine the effect of supplementing roughage (rice straw) and concentrate with inoculants containing four fungal strains (Pleurotus ostreatus, Phanerochaete chrysosporium, Trichoderma reesei and Trichoderma viride) and four bacterial strains (Paenibacillus polymyxa, Bacillus megaterium, Bacillus circulans and Bacillus subtilis), given separately or as a mixture, as a source of exogenous cellulolytic enzymes, on basic rumen parameters in vitro, including digestibility and methane production. A batch culture trial was used to select the best supplements, and a long-term rumen simulation technique (RUSITEC) was used to evaluate the effects of P. chrysosporium, B. subtilis, and a 1 : 1 mixture of these two on dietary component digestibility and fermentation parameters. In the batch culture evaluation, there were significant increases in the organic matter (OM) digestibility, the total gas production expressed as ml/g of dry matter, the OM, the neutral detergent fibre (NDF) and the acid detergent fibre (ADF) of the supplemented rations, as compared to the control, excluding the rations supplemented with T. viride and B. circulans. In the RUSITEC, the ration supplemented with mixed inoculants showed significantly higher digestibility of crude protein, ether extract, NDF and ADF than did the ration supplemented with the P. chrysosporium and B. subtilis inoculants. It can be concluded that the simultaneous use of fungal and bacterial exogenous cellulases on rice straw roughage improves its digestibility, without negative effects on other rumen parameters.

中文翻译：

---

含纤维素外源酶的微生物菌剂作为劣质粗饲料反刍动物日粮饲料添加剂的评价

在反刍动物日粮中添加外源性纤维素分解酶可提高其消化率和饲养价值。本研究的目的是确定用含有四种真菌菌株的接种剂补充粗饲料（稻草）和浓缩物的效果（平菇,黄孢原毛纲,里氏木霉和绿色木霉) 和四种细菌菌株 (多粘类芽孢杆菌,巨大芽孢杆菌,环状芽孢杆菌和枯草芽孢杆菌)，单独或作为混合物给予，作为外源纤维素分解酶的来源，在基本瘤胃参数上体外，包括消化率和甲烷产量。采用分批培养试验来选择最佳补充剂，并采用长期瘤胃模拟技术 (RUSITEC) 来评估P. chrysosporium,枯草芽孢杆菌，以及这两者的 1 : 1 混合物对饮食成分的消化率和发酵参数的影响。在分批培养评估中，有机物 (OM) 消化率、以 ml/g 干物质表示的总产气量、有机物、中性洗涤纤维 (NDF) 和酸性洗涤纤维 (ADF) 显着增加与对照相比，补充口粮的比例，不包括添加了T.绿​​色和B. 循环. 在 RUSITEC 中，添加混合接种剂的日粮对粗蛋白、乙醚提取物、NDF 和 ADF 的消化率显着高于添加混合接种剂的日粮。P. chrysosporium和枯草芽孢杆菌孕育剂。可以得出结论，在稻草粗饲料中同时使用真菌和细菌外源纤维素酶可以提高其消化率，而不会对其他瘤胃参数产生负面影响。