Abstract Rice blast is one of the most destructive diseases of rice and is caused by the infection with rice blast fungus, Magnaporthe oryzae. In the present study, we obtained 12 bacterial isolates from Sichuan basin neutral purplish soil. Among them, the strain named ZW-10 showed significant activity against M. oryzae. Morphological and biochemical studies, along with 16S rDNA sequencing analysis, proved that the strain belongs to Bacillus velezensis. The cell-free culture filtrate of ZW-10 inhibited the growth of M. oryzae, suggesting the presence of active enzymes and secondary metabolites. The experimental results showed that ZW-10 produced cellulase, protease, and peroxidase. The temperature experiment indicated that the secondary metabolisms of ZW-10 remained active at high temperature. In addition, this strain had good acidity resistance. As shown in leaf experiments in vitro as well as field trials, the liquid culture and the cell-free culture filtrate of ZW-10 had significant inhibitory effects on rice blast. In conclusion, ZW-10 can be used as a potential biological agent in the control of rice blast.

中文翻译：

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Bacillus velezensis ZW-10 的分离与评价作为潜在的稻瘟病生物防治剂

摘要 稻瘟病是水稻中最具破坏性的病害之一，由稻瘟病菌Magnaporthe oryzae感染引起。在本研究中，我们从四川盆地中性紫色土壤中获得了 12 株细菌分离物。其中，名为ZW-10的菌株对米曲霉表现出显着的活性。形态学和生化研究以及 16S rDNA 测序分析证明该菌株属于 Bacillus velezensis。ZW-10 的无细胞培养滤液抑制米曲霉的生长，表明存在活性酶和次生代谢物。实验结果表明，ZW-10产生纤维素酶、蛋白酶和过氧化物酶。温度实验表明，ZW-10的次生代谢在高温下仍保持活跃。此外，该菌株具有良好的耐酸性。体外叶片实验和田间试验表明，ZW-10的液体培养和无细胞培养滤液对稻瘟病具有显着的抑制作用。综上所述，ZW-10 可作为防治稻瘟病的潜在生物制剂。