Abstract Juglans regia L. and J. sigillata D. have a long cultivation history in China. With the purpose of detecting interspecific and intraspecific genetic variation, 35 clonal cultivars of J. regia, J. sigillata, J. sigillata×J. regia and J. hopeiensis Hu. (J. mandshurica Maxim. ×J. regia) were selected from a broad geographic range and applied in amplified-fragment length polymorphism (AFLP) analysis. Clear-cut AFLP profiles were produced using 9 EcoR I/Mse I primer combinations. As for J. regia, J. sigillata and their hybrids, the percentages of polymorphic bands (PPBs) averaged 93.86%, 93.94% and 60.46%; the observed number of alleles (Na) averaged 1.5370, 1.6389 and 1.2593; the effective number of alleles (Ne) averaged 1.2582, 1.2543 and 1.1646; Nei's gene diversity (H) scored 0.1560, 0.1556 and 0.0951; Shannon’s information index (I) averaged 0.2402, 0.2459 and 0.1414, respectively. These results implied a moderate level of genetic diversity within the investigated species. The total gene diversity (Ht = 0.1596) can be distributed into intra- (Hs = 0.1356) and inter- (Dst = 0.0240) species gene diversity. The proportion of the distributed genetic diversity among populations (Gst ) was 0.1505, indicating that only 15% of the total gene diversity was attributed to differences between species. The factorial correspondence analysis (FCA) and unweighted pair-group method of arithmetic averages (UPGMA) analysis revealed a close genetic distance between these two species.

中文翻译：

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荧光扩增片段长度多态性揭示的 Juglans regia L. 和 Juglans sigillata D. 的分子遗传变异性

摘要 胡桃（Juglans regia L.）和山核桃（J. sigillata D.）在我国栽培历史悠久。以检测种间和种内遗传变异为目的，对王水、杨树、杨树×J. 35个无性系栽培品种进行了检测。regia 和 J. hopeiensis Hu。(J. mandshurica Maxim. ×J. regia) 选自广泛的地理范围并应用于扩增片段长度多态性 (AFLP) 分析。使用 9 个 EcoR I/Mse I 引物组合产生清晰的 AFLP 配置文件。王豆、银杏及其杂交种的多态带（PPBs）百分比平均为93.86%、93.94%和60.46%；观察到的等位基因数 (Na) 平均为 1.5370、1.6389 和 1.2593；等位基因的有效数目 (Ne) 平均为 1.2582、1.2543 和 1.1646；Nei 的基因多样性（H）得分为 0.1560、0.1556 和 0.0951；香农信息指数 (I) 的平均值分别为 0.2402、0.2459 和 0.1414。这些结果表明所研究物种内的遗传多样性处于中等水平。总基因多样性 (Ht = 0.1596) 可以分为内 (Hs = 0.1356) 和种间 (Dst = 0.0240) 物种基因多样性。种群间分布遗传多样性的比例（Gst）为0.1505，表明只有15%的基因多样性归因于物种间的差异。因子对应分析 (FCA) 和算术平均值的未加权配对组方法 (UPGMA) 分析显示这两个物种之间的遗传距离很近。1596) 可分为内 (Hs = 0.1356) 和间 (Dst = 0.0240) 物种基因多样性。种群间分布遗传多样性的比例（Gst）为0.1505，表明只有15%的总基因多样性归因于物种间的差异。因子对应分析 (FCA) 和算术平均值的未加权配对组方法 (UPGMA) 分析显示这两个物种之间的遗传距离很近。1596) 可分为内 (Hs = 0.1356) 和间 (Dst = 0.0240) 物种基因多样性。种群间分布遗传多样性的比例（Gst）为0.1505，表明只有15%的基因多样性归因于物种间的差异。因子对应分析 (FCA) 和算术平均值的未加权配对组方法 (UPGMA) 分析显示这两个物种之间的遗传距离很近。