Abstract Co-application of titanium dioxide nanoparticles (TiO2 NPs) and plant growth promoting rhizobacteria (PGPR) to promote phytoremediation of Cd contaminated soil was studied. Seedlings of Trifolium repens were exposed to different doses of TiO2 NPs (0, 100, 250, 500 and 1000 mg/kg) and the PGPR, separately and in combination, to investigate the effects on plant growth, Cd uptake and accumulation and chlorophyll content of the plant. Co-application of TiO2 NPs and the PGPR enhanced plant growth and chlorophyll content of T. repens. Cd concentration in roots of T. repens reached 120.3 mg/kg in PGPR + 500 mg/kg TiO2 NPs treatment. Addition of TiO2 NPs to soil significantly increased accumulation capacity of T. repens. The greatest accumulation capacity of 1235 µg/pot was achieved for Cd in PGPR + 500 mg/kg TiO2 NPs treatment. Adverse impacts of using 1000 mg/kg TiO2 NPs were found on plant growth. Co-application of TiO2 NPs and PGPR could reduce the required amounts of TiO2 NPs for phytoremediation of heavy metal polluted soils. Co-application of TiO2 NPs and PGPR promoted growth of T. repens in Cd-contaminated soil and enhanced Cd uptake and accumulation by the plant. Intelligent association of plants, nanomaterials and PGPR have great application prospect in soil remediation.

中文翻译：

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二氧化钛纳米粒子在镉污染土壤植物修复中的应用：接种PGPR的贡献

摘要 研究了二氧化钛纳米颗粒（TiO2 NPs）与植物促生长根际细菌（PGPR）联合应用对Cd污染土壤的植物修复。将三叶草幼苗分别和组合暴露于不同剂量的 TiO2 NPs（0、100、250、500 和 1000 mg/kg）和 PGPR，以研究对植物生长、Cd 吸收和积累以及叶绿素含量的影响的植物。TiO2 NPs 和 PGPR 的共同应用增强了白杨的植物生长和叶绿素含量。在 PGPR + 500 mg/kg TiO2 NPs 处理中，T. repens 根中的 Cd 浓度达到 120.3 mg/kg。向土壤中添加 TiO2 NPs 显着提高了白杨的积累能力。在 PGPR + 500 mg/kg TiO2 NPs 处理中，Cd 的最大积累能力达到 1235 µg/罐。发现使用 1000 mg/kg TiO2 NPs 对植物生长产生不利影响。TiO2 NPs 和PGPR 的共同应用可以减少植物修复重金属污染土壤所需的TiO2 NPs 量。TiO2 NPs 和PGPR 的共同施用促进了白螟在Cd 污染土壤中的生长，并增强了植物对Cd 的吸收和积累。植物、纳米材料与PGPR的智能结合在土壤修复中具有广阔的应用前景。