A new amperometric biosensor was fabricated by means of electropolymerization of L-aspartic acid on a carbon-paste electrode (CPE) for the bioelectrochemical determination of glucose. The electropolymerization process was conducted via cyclic voltammetry (CV). The modified CPE with poly (L-aspartic acid) (PAA) provided free carboxyl groups so as to immobilize the glucose oxidase (GOx), and further, enhanced the electrocatalytic activity of the hydrogen peroxide (H₂O₂). The biosensor displayed both good stability and good bioactivity. The sensitivity of the prepared biosensor was 5.3 µA cm⁻²mM⁻¹. Its linear range extended from 0.05 mM to 1.0 mM, with the low limit of detection (LOD) being 69.2 µM. The Michaelis–Menten constant was found to be 1.17 mM. Furthermore, the biosensor showed good anti-interference ability in relation to dopamine, uric acid, and ascorbic acid. Taken together, these results demonstrate that PAA/CPE is a promising material for the fabrication of glucose biosensor.

通过在碳糊电极（CPE）上进行L-天冬氨酸的电聚合，制备了一种新的电流型生物传感器，用于葡萄糖的生物电化学测定。通过循环伏安法（CV）进行电聚合过程。用聚（L-天冬氨酸）（PAA）改性的CPE提供游离的羧基，从而固定了葡萄糖氧化酶（GOx），并且进一步增强了过氧化氢（H ₂ O ₂）的电催化活性。该生物传感器显示出良好的稳定性和良好的生物活性。制备的生物传感器的灵敏度为5.3 µA cm ^-2 mM ^-1。它的线性范围从0.05 mM扩展到1.0 mM，检测下限（LOD）为69.2 µM。发现米氏常数是1.17 mM。此外，该生物传感器对多巴胺，尿酸和抗坏血酸显示出良好的抗干扰能力。综上所述，这些结果表明PAA / CPE是用于制造葡萄糖生物传感器的有前途的材料。