Abstract Capillary electrophoresis (CE) coupled with mass spectrometry (MS) through electrospray ionization (ESI) is a promising alternative technique to liquid chromatography-ESI-MS (LC-ESI-MS) due to its high separation efficiency and high detection sensitivity. A sensitive and robust interface is essential in CE-ESI-MS. Continued development of CE-ESI-MS interfaces in the last decade, including junction-at-the-tip interfaces and sheathless interfaces, has improved the sensitivity and robustness of CE-ESI-MS significantly. The limited loading capacity of CE, one of major reasons that limits the utility of CE as a routine separation method, has also been addressed effectively by the use of in-capillary sample preconcentration techniques, such as transient CITP/CZE and dynamic pH junction. CE-ESI-MS could yield higher sensitivity as compared with the conventional LC-ESI-MS, and, therefore, is capable of identifying more proteins and peptides when the sample amount is very limited, such as single cell analysis. To improve the protein sequence coverage, CE-ESI-MS can also be used as a complementary technique to LC-ESI-MS, or combined with reversed phase LC to form a two dimensional separation technique. CE-ESI-MS is also effective in quantifying targeted peptides/proteins in complex bio-matrix.

中文翻译：

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高灵敏度和稳健的毛细管电泳-电喷雾电离-质谱：接口、预浓缩技术和应用

摘要 毛细管电泳 (CE) 与通过电喷雾电离 (ESI) 的质谱 (MS) 联用是一种很有前景的液相色谱-ESI-MS (LC-ESI-MS) 替代技术，因为它具有高分离效率和高检测灵敏度。在 CE-ESI-MS 中，灵敏而稳定的接口是必不可少的。在过去十年中，CE-ESI-MS 接口的持续发展，包括尖端结接口和无鞘接口，显着提高了 CE-ESI-MS 的灵敏度和稳健性。CE 的有限负载能力是限制 CE 作为常规分离方法效用的主要原因之一，也已通过使用毛细管内样品预浓缩技术（例如瞬态 CITP/CZE 和动态 pH 连接点）得到有效解决。与传统的 LC-ESI-MS 相比，CE-ESI-MS 可以产生更高的灵敏度，因此，当样品量非常有限时，例如单细胞分析，能够识别更多的蛋白质和肽。为提高蛋白质序列覆盖率，CE-ESI-MS还可作为LC-ESI-MS的补充技术，或与反相LC结合形成二维分离技术。CE-ESI-MS 还可有效定量复杂生物基质中的目标肽/蛋白质。或与反相LC结合形成二维分离技术。CE-ESI-MS 还可有效定量复杂生物基质中的目标肽/蛋白质。或与反相LC结合形成二维分离技术。CE-ESI-MS 还可有效定量复杂生物基质中的目标肽/蛋白质。