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Impact of Microwave Plasma Torch on the Yeast Candida glabrata
Applied Sciences ( IF 2.838 ) Pub Date : 2020-08-11 , DOI: 10.3390/app10165538
Kristína Trebulová , František Krčma , Zdenka Kozáková , Petra Matoušková

Recently, various cold plasma sources have been tested for their bactericidal and fungicidal effects with respect to their application in medicine and agriculture. The purpose of this work is to study the effects of a 2.45 GHz microwave generated plasma torch on a model yeast example Candida glabrata. The microwave plasma was generated by a surfatron resonator, and pure argon at a constant flow rate of 5 Slm was used as a working gas. Thanks to a high number of active particles generated in low-temperature plasma, this type of plasma has become highly popular, especially thanks to its bactericidal effects. However, its antimycotic effects and mechanisms of fungal inactivation are still not fully understood. Therefore, this study focuses on the antifungal effects of the microwave discharge on Candida glabrata. The main focus is on the measurement and evaluation of changes in inactivation effects caused by varying initial concentration of Candida glabrata cells, applied microwave power and exposure time. The discharge was applied on freshly inoculated colonies of Candida glabrata spread on the agar plates and its inhibitory effects were observed in the form of inhibition zones formed after the subsequent cultivation.

中文翻译:

微波等离子炬对酵母光滑念珠菌的影响

近来,已经对各种冷血浆源就其在医学和农业中的应用进行了杀菌和杀真菌作用的测试。这项工作的目的是研究2.45 GHz微波产生的等离子炬对模型酵母示例光滑念珠菌的影响。微波等离子体由超音速共振器产生,并且以5 Slm的恒定流量使用纯氩气作为工作气体。由于在低温等离子体中产生了大量的活性颗粒,这种等离子体已变得非常流行,特别是由于其具有杀菌作用。但是,其抗霉菌作用和真菌失活的机制仍未完全了解。因此,本研究着眼于微波放电对真菌的抗真菌作用。光滑念珠菌。主要关注点是测量和评估因改变光滑念珠菌细胞的初始浓度,施加的微波功率和暴露时间而导致的灭活效果的变化。将放电物施加在散布在琼脂板上的新鲜接种的光滑念珠菌菌落上,并在随后的培养后形成的抑制区的形式观察到其抑制作用。
更新日期:2020-08-11
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