Biotransformation of fatty acids from renewable wastewater as feedstock to value-added chemicals is a fascinating commercial opportunity. α,ω-dicarboxylic acids (DCAs) are building blocks in many industries, such as polymers, cosmetic intermediates, and pharmaceuticals, and can be obtained by chemical synthesis under extreme conditions. However, biological synthesis can replace the traditional chemical synthesis using cytochrome P450 enzymes to oxidize fatty acids to DCAs. Saccharomyces cerevisiae BY(2R)/pYeDP60-CYP52A17^SS (BCM), a transgenic strain expressing the galactose-inducible CYP52A17^SS cytochrome P450 enzyme, was able to grow in a coconut milk factory wastewater (CCW) medium and produced 12-hydroxydodecanoic acid (HDDA) and 1,12-dodecanedioic acid (DDA). The supplementation of CCW with 10 g/L yeast extract and 20 g/L peptone (YPCCW) markedly increased the yeast growth rate and the yields of 12-HDDA and 1,12-DDA, with the highest levels of approximately 60 and 38 µg/L, respectively, obtained at 30 °C and pH 5. The incubation temperature and medium pH strongly influenced the yeast growth and 1,12-DDA yield, with the highest 1,12-DDA formation at 30 °C and pH 5–5.5. Hence, the S. cerevisiae BCM strain can potentially be used for producing value-added products from CCW.

中文翻译：

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重组表达啤酒酵母的CYP52A17SS从椰奶厂废水中全细胞生物转化1,12-十二烷二酸

从可再生废水作为原料将脂肪酸生物转化为增值化学品是一个引人入胜的商业机会。α，ω-二羧酸（DCA）是许多行业的基础，例如聚合物，化妆品中间体和药物，并且可以在极端条件下通过化学合成获得。然而，生物合成可以代替传统的化学合成，利用细胞色素P450酶将脂肪酸氧化为DCA。酿酒酵母BY（2R）/ pYeDP60-CYP52A17 ^SS（BCM），一种表达半乳糖诱导型CYP52A17 ^SS的转基因菌株细胞色素P450酶能够在椰奶工厂废水（CCW）培养基中生长，并产生12-羟基十二烷酸（HDDA）和1,12-十二烷二酸（DDA）。在CCW中添加10 g / L酵母提取物和20 g / L蛋白ept（YPCCW）可以显着提高酵母的生长速度和12-HDDA和1,12-DDA的产量，最高含量约为60和38 µg / L分别在30°C和pH 5下获得。孵育温度和中等pH值强烈影响酵母的生长和1,12-DDA产量，在30°C和pH 5–下形成的1,12-DDA最高。 5.5。因此，酿酒酵母BCM菌株可以潜在地用于从CCW生产增值产品。