The introduction of a strain or consortium has often been considered as a potential solution to restore microbial ecosystems. Extensive research on the skin microbiota has led to the development of probiotic products (with live bacterial strains) that are likely to treat dysbiosis. However, the effects of such introductions on the indigenous microbiota have not yet been investigated. Here, through a daily application of Lactobacillus reuteri DSM 17938 on volunteers’ forearm skin, we studied in vivo the impact of a probiotic on the indigenous skin bacterial community diversity using Terminal-Restriction Fragment Length Polymorphism (T-RFLP) for 3 weeks. The results demonstrate that Lactobacillus reuteri DSM 17938 inoculum had a transient effect on the indigenous community, as the resilience phenomenon was observed within the skin microbiota. Moreover, Lactobacillus reuteri DSM 17938 monitoring showed that, despite a high level of detection after 2 weeks of application, thereafter the colonization rate drops drastically. The probiotic colonization rate was correlated significantly to the effect on the indigenous microbial community structure. These preliminary results suggest that the success of probiotic use and the potential health benefits resides in the interactions with the human microbiota.

中文翻译：

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人类皮肤细菌对益生菌的反应（罗伊氏乳杆菌DSM 17938）介绍。

菌株或联合体的引入通常被认为是恢复微生物生态系统的潜在解决方案。对皮肤微生物群的广泛研究已导致开发可能治疗营养不良的益生菌产品（带有活细菌菌株）。但是，尚未研究这种引入对本地微生物的影响。在这里，通过每天在志愿者的前臂皮肤上使用罗伊氏乳杆菌DSM 17938，我们使用末端限制性片段长度多态性（T-RFLP）进行了3周的体内益生菌对本地皮肤细菌群落多样性的影响研究。结果表明罗伊氏乳杆菌DSM 17938接种物对土著社区具有暂时性影响，因为在皮肤微生物群中观察到了弹性现象。此外，罗伊氏乳杆菌DSM 17938的监测表明，尽管在施用2周后检出率很高，但随后的定殖率却急剧下降。益生菌的定殖率与对土著微生物群落结构的影响显着相关。这些初步结果表明，益生菌使用的成功和潜在的健康益处在于与人类微生物群的相互作用。