The aim of the present study was to evaluate the comparative effects of red (660‐nm) and near‐infrared (780‐nm) low‐level laser therapy (LLLT) on viability, mitochondrial activity, morphology and gene expression of growth factors on Schwann cells (SC). ST88‐14 cells were grown in RPMI 1640 with 10 mM of HEPES, 2 mM of glutamine, 10% fetal bovine serum and 1% antibiotic‐antimycotic solution at 37°C in humidified atmosphere of 5% CO2. Cells were detached with trypsin and centrifugated at 231 g for 5 min at 10°C, and the pellet (8 × 104 cells/tube) was irradiated at the bottom of 50 ml polypropylene tube with a Twin‐Laser system (660 and 780 nm, 40 mW, 1 mW cm−2, 3.2 and 6.4 J, 80 and 160 J cm−2 with 80 and 160 s). After 1, 3 and 7 days, the analysis was performed. After irradiation, the SC increase mitochondrial activity, gene expression of the neural growth factors NGF and BDNF, and cell migration and increase the G2/M cells. SC showed neuronal morphology, normal F‐actin cytoskeleton organization and positive labeling for S100. PBM increased metabolic activity, mitosis and gene expression when irradiated with red and infrared LLLT. An increase in cell migration was obtained when irradiated with infrared LLLT.

中文翻译：

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660-nm 和 780-nm 激光疗法对 ST88-14 雪旺氏细胞的影响

本研究的目的是评估红色 (660 nm) 和近红外 (780 nm) 低强度激光疗法 (LLLT) 对生长因子的活力、线粒体活性、形态和基因表达的比较影响。施万细胞（SC）。ST88-14 细胞在含有 10 mM HEPES、2 mM 谷氨酰胺、10% 胎牛血清和 1% 抗生素抗真菌溶液的 RPMI 1640 中于 37°C 和 5% CO2 的湿润气氛中生长。用胰蛋白酶分离细胞并在 10°C 下以 231 g 离心 5 分钟，用双激光系统（660 和 780 nm）在 50 ml 聚丙烯管底部照射沉淀（8 × 104 个细胞/管） 、40 mW、1 mW cm-2、3.2 和 6.4 J、80 和 160 J cm-2，80 和 160 秒）。1、3、7天后进行分析。照射后，SC增加线粒体活性，神经生长因子 NGF 和 BDNF 的基因表达，以及细胞迁移和增加 G2/M 细胞。SC 显示神经元形态、正常的 F-肌动蛋白细胞骨架组织和 S100 的阳性标记。当用红色和红外线 LLLT 照射时，PBM 增加了代谢活性、有丝分裂和基因表达。当用红外线 LLLT 照射时，获得了细胞迁移的增加。