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Function and application of the Eutrema salsugineum PHT1;1 gene in phosphate deficiency stress.
Plant Biology ( IF 4.2 ) Pub Date : 2020-08-11 , DOI: 10.1111/plb.13169 S Yang 1 , Y Zhao 1 , J Wang 1
中文翻译：
Eutrema salsugineum PHT1;1基因在缺磷胁迫中的功能和应用。
更新日期：2020-08-11
Plant Biology ( IF 4.2 ) Pub Date : 2020-08-11 , DOI: 10.1111/plb.13169 S Yang 1 , Y Zhao 1 , J Wang 1
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- Low availability of inorganic phosphate (Pi) in soil is often a limiting factor for plant growth and productivity. The Pi transporter plays an important role in the absorption and utilization of phosphorus in plants. Eutrema salsugineum shows strong tolerance under Pi stress conditions, but the function of the E. salsugineum PHT1 genes has not yet been systematically studied.
- This study isolated a phosphate transporter gene (EsPHT1;1) from the halophyte E. salsugineum and functionally characterized it in the herbaceous model plant, Arabidopsis thaliana, and in an important oil crop species, soybean (Glycine max (L.) Merr.).
- Under Pi deficient conditions, transgenic Arabidopsis and transgenic soybean grew better and exhibited significant improvement in root growth, biomass accumulation and seed yield compared with wild‐type (WT) plants. These phenotypic enhancements were more apparent under inadequate Pi conditions than under sufficient or no Pi conditions, which is in agreement with the observation that the transgenic plants accumulated higher amounts of Pi and total P in shoots and roots than WT plants only when inadequate Pi was supplied.
- The results of the present study indicate that overexpression of EsPHT1;1 can efficiently enhance the growth and reproductive performance of both Arabidopsis and soybean plants challenged by low P stress, which results confirm the important role of PHT1;1 in dealing with Pi deficiency.
中文翻译：
Eutrema salsugineum PHT1;1基因在缺磷胁迫中的功能和应用。
土壤中无机磷酸盐 (Pi) 的利用率低通常是植物生长和生产力的限制因素。 Pi转运蛋白在植物对磷的吸收和利用中发挥着重要作用。 Eutrema salsugineum在Pi胁迫条件下表现出较强的耐受性，但E. salsugineum PHT1基因的功能尚未得到系统研究。
本研究从盐生植物E. salsugineum中分离出磷酸盐转运蛋白基因 ( EsPHT1;1 )，并在草本模式植物拟南芥和重要油料作物大豆 ( Glycine max (L.) Merr.) 中对其进行功能表征。 。
在缺磷条件下，与野生型（WT）植物相比，转基因拟南芥和转基因大豆生长得更好，并且在根系生长、生物量积累和种子产量方面表现出显着改善。这些表型增强在 Pi 不足的条件下比在 Pi 充足或无 Pi 的条件下更为明显，这与观察结果一致，即仅当 Pi 供应不足时，转基因植物在芽和根中积累的 Pi 和总 P 量才高于 WT 植物。
本研究结果表明， EsPHT1;1的过表达可以有效增强低磷胁迫下的拟南芥和大豆植物的生长和繁殖性能，这一结果证实了PHT1;1在应对低磷胁迫中的重要作用。
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