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Ectopic expression of common bean ERF transcription factor PvERF35 promotes salt stress tolerance in tobacco.
Plant Biology ( IF 4.2 ) Pub Date : 2020-08-10 , DOI: 10.1111/plb.13167 M Kavas 1 , G Gökdemir 1 , Z Seçgin 1 , A Bakhsh 2
中文翻译：
菜豆ERF转录因子PvERF35的异位表达促进烟草耐盐胁迫。
更新日期：2020-08-10
Plant Biology ( IF 4.2 ) Pub Date : 2020-08-10 , DOI: 10.1111/plb.13167 M Kavas 1 , G Gökdemir 1 , Z Seçgin 1 , A Bakhsh 2
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- In the present study, a TINY‐like AP2/ERF gene, PvERF35i, was amplified from common bean (Phaseolus vulgaris L.), cloned and functionally characterized by overexpressing in tobacco cv. Petite havana.
- Transgenic plants overexpressing PvERF35 were generated using Agrobacterium‐mediated transformation and used to evaluate the possible roles of the transgene under salt stress conditions. Evaluation of transgenics was completed using both molecular and biochemical analysis.
- PCR, Southern blot and RT‐qPCR assays revealed the correct integration and enhanced expression of the transgene. Physiological and biochemical analysis of transgenic plants showed their better performance compared to the wild type in terms of germination and survival rates and root and shoot growth under salt stress treatment (200 mM NaCl). Having a high concentration of proline, APX and POX, the PvERF35 overexpressed plants were physiologically and morphologically less affected by salt stress application. In silico promoter analysis of the PvERF35 gene led to identification of important cis‐regulatory elements, MYB, MYC and TGACG‐motif, annotated with salt response of plants. The protein–protein interaction network showed that there was a strong association between ABC transporter proteins and PvERF35 protein. Salt stress‐related miRNA, miRNA156 and miRNA159, targeting PvERF35 were identified using in silico target finding analysis.
- These findings suggest that PvERF35 functions as a stress‐responsive transcription factor in differential modulation of salt stress tolerance and may have applications in the engineering of economically important crops.
中文翻译：
菜豆ERF转录因子PvERF35的异位表达促进烟草耐盐胁迫。
在本研究中，从普通菜豆（ Phaseolus vulgaris L.）中扩增出一个 TINY 样 AP2/ERF 基因PvERF35i ，并通过在烟草品种中过度表达进行克隆和功能表征。娇小的哈瓦那。
使用农杆菌介导的转化产生过表达PvERF35的转基因植物，并用于评估转基因在盐胁迫条件下的可能作用。使用分子和生化分析完成转基因评估。
PCR、Southern blot 和 RT-qPCR 检测显示转基因的正确整合和增强的表达。转基因植物的生理生化分析表明，与野生型相比，在盐胁迫处理（200 mM NaCl）下，转基因植物在发芽率、成活率以及根和芽生长方面表现出更好的性能。 PvERF35过表达植物具有高浓度的脯氨酸、APX和POX，其生理和形态受盐胁迫的影响较小。 PvERF35基因的计算机启动子分析鉴定出重要的顺式调控元件 MYB、MYC 和 TGACG 基序，并用植物的盐响应进行注释。蛋白质-蛋白质相互作用网络表明 ABC 转运蛋白和 PvERF35 蛋白之间存在很强的关联。使用计算机目标发现分析鉴定了靶向PvERF35的盐胁迫相关 miRNA、miRNA156 和 miRNA159。
这些发现表明， PvERF35在盐胁迫耐受性的差异调节中充当胁迫响应转录因子，并且可能在具有重要经济意义的作物工程中得到应用。
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