Obesity stimulates the macrophage infiltration and senescence state in adipose tissues of humans and rodents. The adipogenesis capacity of Japanese Black cattle (Wagyu) is higher than that of Holsteins. We hypothesized that breed differences between Wagyu and Holsteins may affect the level of macrophage infiltration and senescence state in adipose tissues. The macrophage infiltration, senescence marker gene expression and activity of senescence‐associated β‐galactosidase (SA‐βgal) in visceral and intramuscular adipose tissue of Wagyu were higher than those of Holsteins. In contrast, there were no differences in macrophage infiltration, senescence marker gene expression and activity of SA‐βgal in subcutaneous adipose tissue between the breeds. Expression of p53 gene, the master regulator of macrophage infiltration and senescence state, in visceral and intramuscular adipose tissue of Wagyu was higher than that of Holsteins. In contrast, there was no difference in the expression of p53 gene in subcutaneous adipose tissue between the breeds. These results suggest that breed differences in macrophage infiltration and senescence state in adipose tissues of Wagyu and Holsteins are affected by p53 expression.

中文翻译：

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和牛和荷斯坦牛脂肪组织中巨噬细胞浸润和衰老状态的品种差异。

肥胖会刺激人类和啮齿动物脂肪组织中的巨噬细胞浸润和衰老状态。日本黑牛 (Wagyu) 的脂肪形成能力高于荷斯坦牛。我们假设和牛和荷斯坦牛之间的品种差异可能会影响脂肪组织中巨噬细胞浸润水平和衰老状态。和牛内脏和肌内脂肪组织中的巨噬细胞浸润、衰老标记基因表达和衰老相关β-半乳糖苷酶（SA-βgal）活性均高于荷斯坦牛。相比之下，不同品种之间皮下脂肪组织中的巨噬细胞浸润、衰老标记基因表达和 SA-βgal 活性没有差异。p53 基因的表达，巨噬细胞浸润和衰老状态的主要调节因子，和牛的内脏和肌肉脂肪组织中的 含量高于荷斯坦牛。相反，不同品种皮下脂肪组织中p53基因的表达没有差异。这些结果表明，和牛和荷斯坦牛脂肪组织中巨噬细胞浸润和衰老状态的品种差异受 p53 表达的影响。