Porphyrin‐conjugated silver‐coated gold nanostars for ultrasensitive detection and multiplexing,Journal of Raman Spectroscopy

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Porphyrin‐conjugated silver‐coated gold nanostars for ultrasensitive detection and multiplexing
Journal of Raman Spectroscopy ( IF 2.4 ) Pub Date : 2020-08-11 , DOI: 10.1002/jrs.5963
Leandro N. Benavides _{1,

2} , M. Sergio Moreno ₃ , Daniel H. Murgida _{1,

2} , María A. Castro _{1,

2}

Affiliation

Here, we propose the use of silver‐coated gold nanostars as the basis for a multiplex sensing system based on surface‐enhanced Raman scattering (SERS)/surface‐enhanced resonance Raman scattering (SERRS) ultrasensitive detection of porphyrins using a 532 nm laser excitation. By modifying the synthetic parameters, we were able to shift the plasmon peak to an optimized position for SERS enhancement of 540 nm for silver‐coated gold nanostars, estimating a SERS enhancement factor around 4.6 × 10⁵. Furthermore, we show high‐resolution transmission electron microscopy (HR‐TEM) images and electron energy‐loss (EEL) spectra of silver‐coated gold nanostars, which provide solid experimental proof on the proposed silver coating mechanism. Finally, we compare the multiplex performance of common reporters such as cyanines and malachite green with the performance of covalently bound porphyrins and show the superiority of the latter. We studied the multiplex detection capabilities of the system with three different porphyrins. By deconvoluting a mixture spectrum into individual porphyrin spectra, we were able to determine the relative concentration of porphyrins with an average error of 3.1%.

中文翻译：

卟啉偶联的银包金纳米星，用于超灵敏的检测和多路复用

在此，我们建议使用镀银金纳米星作为基于532 nm激光激发的卟啉表面增强拉曼散射（SERS）/表面增强共振拉曼散射（SERRS）超灵敏检测的多重传感系统的基础。通过修改合成参数，我们能够将等离激元峰移动到最佳位置，以使镀银金纳米星的SERS增强到540 nm，估计SERS增强因子约为4.6×10 ⁵。此外，我们显示了镀银金纳米星的高分辨率透射电子显微镜（HR-TEM）图像和电子能量损失（EEL）光谱，为提出的镀银机理提供了坚实的实验依据。最后，我们将花青和孔雀绿等常见报道分子与共价结合卟啉的表现进行了比较，并显示了后者的优越性。我们研究了使用三种不同卟啉的系统的多重检测能力。通过将混合物光谱解卷积成各个卟啉光谱，我们能够确定卟啉的相对浓度，平均误差为3.1％。

更新日期：2020-08-11

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