Microglia polarization is associated with the pathogenesis of depression. A previous study shows that long non‐coding RNA uc.80‐ is down‐regulated in the hippocampus of depressed rats. Thus, this article aims to investigate the role of uc.80‐ in microglia polarization in depression. We first established depression model rats by chronic unpredictable mild stress (CUMS) regiment. We found that hippocampus of depressed rats exhibited an increase of M1 microglias and a decrease of M2 microglias. uc.80‐ was down‐regulated in hippocampus of depressed rats. Furthermore, the detection of behaviouristics of depressed rats showed that uc.80‐ overexpression alleviated depression of rats. In addition, uc.80‐ overexpression promoted M2 polarization of microglias in vivo and in vitro. uc.80‐ overexpression led to a decrease in apoptosis of hippocampal neurons in vivo and in vitro. In conclusion, our study confirms that lncRNA uc.80‐ overexpression ameliorates depression in rats by promoting M2 polarization of microglias. Thus, our work suggests that uc.80‐ may be a target gene for depression treatment.

中文翻译：

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长链非编码RNA uc.80-过表达促进小胶质细胞M2极化改善大鼠抑郁症

小胶质细胞极化与抑郁症的发病机制有关。之前的一项研究表明，长链非编码 RNA uc.80- 在抑郁大鼠的海马体中被下调。因此，本文旨在研究 uc.80- 在抑郁症小胶质细胞极化中的作用。我们首先通过慢性不可预测轻度应激（CUMS）团建立抑郁模型大鼠。我们发现抑郁大鼠的海马表现出 M1 小胶质细胞的增加和 M2 小胶质细胞的减少。uc.80-在抑郁大鼠的海马中下调。此外，对抑郁大鼠行为学的检测表明，uc.80-过表达减轻了大鼠的抑郁。此外，uc.80-过表达促进了体内和体外小胶质细胞的M2极化。加州大学 80-过表达导致体内外海马神经元凋亡减少。总之，我们的研究证实 lncRNA uc.80-过表达通过促进小胶质细胞的 M2 极化来改善大鼠的抑郁症。因此，我们的工作表明 uc.80- 可能是抑郁症治疗的靶基因。