当前位置: X-MOL 学术Angew. Chem. Int. Ed. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Sharp Switching of DNAzyme Activity through the Formation of a CuII -Mediated Carboxyimidazole Base Pair.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-08-10 , DOI: 10.1002/anie.202009579
Yusuke Takezawa 1 , Lingyun Hu 1 , Takahiro Nakama 1 , Mitsuhiko Shionoya 1
Affiliation  

DNAzymes are widely used as functional units for creating DNA‐based sensors and devices. Switching of DNAzyme activity by external stimuli is of increasing interest. Herein we report a CuII‐responsive DNAzyme rationally designed by incorporating one of the most stabilizing artificial metallo‐base pairs, a CuII‐mediated carboxyimidazole base pair (ImC‐CuII‐ImC), into a known RNA‐cleaving DNAzyme. Cleavage of the substrate was suppressed without CuII, but the reaction proceeded efficiently in the presence of CuII ions. This is due to the induction of a catalytically active structure by ImC‐CuII‐ImC pairing. The on/off ratio was as high as 12‐fold, which far exceeds that of the previously reported DNAzyme with a CuII‐mediated hydroxypyridone base pair. The DNAzyme activity can be regulated specifically in response to CuII ions during the reaction through the addition, removal, or reduction of CuII. This approach should advance the development of stimuli‐responsive DNA systems with a well‐defined sharp switching function.

中文翻译:

通过形成CuII介导的羧咪唑碱基对,DNA酶活性急剧变化。

DNAzyme被广泛用作创建基于DNA的传感器和设备的功能单元。外部刺激对DNAzyme活性的转换引起了越来越多的关注。本文我们报告一个的Cu II响应性DNA核酶合理通过将最稳定人工金属-碱基对中的一个设计中,铜II介导的carboxyimidazole碱基对(IM ç -Cu II -Im Ç),进入一个已知的RNA切割核酶。在没有Cu II的情况下抑制了基体的裂解,但是在Cu II离子的存在下反应有效地进行了。这是由于Im C ‐Cu II ‐Im诱导了催化活性结构C配对。开/关比高达12倍,远远超过了先前报道的带有Cu II介导的羟基吡啶酮碱基对的DNAzyme。通过添加,去除或还原Cu II,可以在反应过程中响应Cu II离子来具体调节DNAzyme的活性。这种方法应促进具有明确定义的快速切换功能的刺激响应性DNA系统的开发。
更新日期:2020-08-10
down
wechat
bug