The present work reports the spectroscopic and theoretical evaluation of the interaction between calf-thymus DNA (CT-DNA) and free-base meso-tetra-(ruthenated) porphyrin (H₂RuTPyP) or its corresponding Zn(II) complex (ZnRuTPyP). Spectroscopic measurements (UV–vis, circular dichroism and steady-state fluorescence emission) combined with theoretical molecular docking calculations suggest that Ru(II)-porphyrins interact with the DNA backbone by external mode via electrostatic forces. In addition, gel electrophoresis analysis demonstrate that these porphyrins promote efficient plasmidial DNA photocleavage upon white-light irradiation conditions, indicating H₂RuTPyP and ZnRuTPyP as potential candidates for photodynamic therapy.

本工作报告了小牛胸腺DNA（CT-DNA）与游离碱内消旋-四-（钌）卟啉（H ₂ RuTPyP）或其相应的Zn（II）络合物（ZnRuTPyP）之间相互作用的光谱学和理论评价。。光谱测量（紫外可见光，圆二色性和稳态荧光发射）与理论分子对接计算的结合表明，Ru（II）-卟啉通过静电力通过外部模式与DNA骨架相互作用。此外，凝胶电泳分析表明，这些卟啉在白光照射条件下可促进有效的质粒DNA光裂解，表明H ₂ RuTPyP和ZnRuTPyP可作为光动力疗法的潜在候选物。