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Rapid and simple As(III) quantification using a turbidimetric test for the monitoring of microbial arsenic bio-transformation.
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2020-08-11 , DOI: 10.1016/j.mimet.2020.106026
Caroline Michel 1 , Catherine Joulian 1 , Hafida Tris 1 , Flavie Charnois 1 , Fabienne Battaglia-Brunet 1
Affiliation  

A turbidimetric test for rapid quantification of As(III) (detection limit of 3 mg/L, quantification range of 10–100 mg/L) in liquid growth medium was developed for assessing and monitoring microbial As(III)-oxidizing and As(V)-reducing activities. This test is based on As(III) chelation with pyrrolidine dithiocarbamate followed by spectrometric measurement of absorbance, and was validated by comparison with AAS quantification of As after As(III)/As(V) separation.



中文翻译:

快速,简单的As(III)定量使用浊度法监测微生物砷的生物转化。

为了评估和监测微生物中As(III)的氧化和As(III)的含量,开发了一种比浊法,用于快速定量液体生长培养基中的As(III)(检出限为3 mg / L,定量范围为10-100 mg / L)。五)减少活动。该测试基于吡咯烷二硫代氨基甲酸酯与As(III)的螯合,然后进行吸光度的光谱测定,并且通过与As(III)/ As(V)分离后的AAS定量比较进行了验证。

更新日期:2020-08-29
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