Acinetobacter baumannii causes serious multidrug resistant nosocomial infections around the world. This comprehensive comparative study was designed to assess the effect of temperature (30, 37 and 42 °C), incubation (aerobic and microaerobic) condition and selective [CHROMagar Acinetobacter (CHR) and Leeds Acinetobacter Medium (LAM)] and non-selective [Modified Karmali Agar (MKA)] growth media on the enhanced recovery of A. baumannii from a variety of water (agricultural, recreational, raw drinking intake source, pre-chlorinated and post-chlorinated wastewater effluent) samples spiked with a known number of A. baumannii cells. After spiking each water type with a known number of cells in 10 mL volume, the sample was passed through a membrane filter (pore size 0.45 μm) and filters were placed on different selective media plates and subjected to incubate at various incubation conditions. The results reported in this study show that for all water types tested (except post-chlorinated wastewater effluent), LAM was the most effective selective growth medium in combination with variable temperature and incubation conditions for yielding high recovery rates of A. baumannii cells. Overall, A. baumannii showed that it has a high adaptive capacity to grow on selective and non-selective growth media at different temperature and incubation conditions. The data described in this study suggest that no single incubation condition and growth media would efficiently recover A. baumannii from all environmental water types tested. This data also indicate that selective growth media and incubation condition can significantly affect the recovery of A. baumannii. Differences in recovery of A. baumannii observed in this study which appeared to be dependent on the temperature and environmental characteristics of incubation as well as the sample type, suggest the need for caution when comparing recovery using different protocols.

鲍曼不动杆菌在世界范围内引起严重的多药耐药医院感染。这项全面的比较研究旨在评估温度（30、37和42°C），孵育（有氧和微有氧）条件以及选择性[CHROMagar不动杆菌（CHR）和利兹不动杆菌培养基（LAM）]和非选择性[改良的Karmali琼脂（MKA）生长培养基，涉及从已知标有已知数量A的多种水（农业，娱乐，生饮，饮用水，预氯化和后氯化废水）中提高鲍曼不动杆菌的回收率鲍曼尼细胞。用10 mL体积的已知数量的细胞加标每种水类型后，将样品通过膜滤器（孔径0.45μm），并将滤器置于不同的选择性培养基板上，并在各种温育条件下进行温育。这项研究报告的结果表明，对于所有测试的水类型（氯化后废水除外），LAM是最有效的选择性生长培养基，具有可变的温度和孵育条件，可提高鲍曼不动杆菌的回收率。总体而言，鲍曼不动杆菌结果表明，它具有在不同温度和孵育条件下在选择性和非选择性生长培养基上生长的高适应能力。这项研究中描述的数据表明，没有一个单独的孵化条件和生长培养基可以从所有测试的环境水类型中有效回收鲍曼不动杆菌。该数据还表明，选择性生长培养基和孵育条件可以显着影响鲍曼不动杆菌的恢复。在这项研究中观察到的鲍曼不动杆菌的回收率差异似乎取决于孵育的温度和环境特征以及样品类型，这表明在使用不同方案比较回收率时需要谨慎。