The biotransformation ability of the organism is the result of organ-specific responses. This paper presents a molecular and biochemical approach to elucidate the biotransformation mechanisms in different organs of Prochilodus lineatus induced at 6, 24, and 96 h after a benzo[a]pyrene (B[a]P) injection. The induction in cyp1a transcription showed an organ-specific intensity at every tested time time. The EROD (ethoxyresorufin-O-deethylase) activity increased rapidly (6 h) in the liver and the kidney; the gills and the brain showed an increase at 24 h; and the gills demonstrated the highest activity among all the organs tested. There was no increase in glutathione S-transferase (GST) activity or lipoperoxidation. The decreased hepatic glutathione content (GSH) may be due to its role as an antioxidant. B[a]P was detected in the bile, confirming the xenobiotic efflux from the metabolizing organs. The gills, liver, brain, and kidney of P. lineatus presented an integrated mechanism to deal with the xenobiotic biotransformation.

生物体的生物转化能力是器官特异性反应的结果。本文提出了一种分子和生化方法，阐明了在注射苯并[a] py（B [a] P）后6、24和96小时，线虫的不同器官中的生物转化机制。cyp1a转录的诱导显示每个测试时间的器官特异性强度。肝脏和肾脏中的EROD（乙氧基异色蛋白O-脱乙基酶）活性迅速增加（6小时）。24小时时h和大脑显示增加；ill表现出所有被测器官中最高的活性。谷胱甘肽S-转移酶（GST）活性或脂过氧化没有增加。肝谷胱甘肽含量（GSH）降低可能是由于其作为抗氧化剂的作用。在胆汁中检测到B [a] P，确认异物从代谢器官流出。P. lineatus的腮，肝，脑和肾呈现了一种综合机制来应对异种生物的生物转化。