The effects of regular aerobic exercise training on blood nitric oxide levels and oxidized LDL and the role of eNOS intron 4a/b polymorphism.,Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

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The effects of regular aerobic exercise training on blood nitric oxide levels and oxidized LDL and the role of eNOS intron 4a/b polymorphism.
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease ( IF 4.2 ) Pub Date : 2020-08-11 , DOI: 10.1016/j.bbadis.2020.165913
Yeliz Yol ₁ , Faruk Turgay ₂ , Oya Yigittürk ₁ , Semih Aşıkovalı ₃ , Burak Durmaz ₃

Affiliation

Background

Nitric oxide (NO), oxidized LDL (OxLDL) and endothelial nitric oxide synthase intron 4a/b polymorphism (eNOSP) are related to atherosclerosis (AS). The present study investigated the effects of regular aerobic exercise training on the mentioned risk factors as well as blood lipids and lipoproteins (BLLPs) and the role of eNOSP, which is unclear.

Methods

The study was participated by 46 well trained male soccer referees as the athletic group (AG, age; 23.26 ± 2.84 years) and 43 sedentary controls (CG, age; 23.16 ± 3.28 years). Yoyo intermittent endurance (Yoyo IE-2 test) was performed to measure aerobic endurance levels of the participants. Serum NO, eNOS and oxidized LDL (OxLDL) levels (by ELISA method) and total oxidant /antioxidant status ratio (/TOS/TAS) as oxidative stress (OS) index (OSI) and BLLPs levels were determined. eNOSP was identified from genomic DNA samples with VNTR analysis.

Results

There is no significant difference between AG and CG including the genotype groups for NO, eNOS and BLLPs and eNOSP has no role. However, AG's NO (29%, p > .05) and TAS levels were significantly higher (p = .001) than those of CG, whereas OSI (p = .001) and OxLDL (p = .011) values were significantly lower. On the other hand, NO value of the athletic bb group was 29% higher compared with the control and the a carrier (aC = aa + ab) group.

Conclusions

These findings suggest that regular aerobic exercise improves blood NO levels and antioxidant capacity, while decreasing OS levels including OxLDL, but not eNOS and BLLPs in the athletes. Although the polymorphism does not have a modifying effect on these effects, bb genotype group may benefit more from training for NO than aC group due to genetic tendency.

中文翻译：

定期有氧运动训练对血液中一氧化氮水平和氧化LDL的影响以及eNOS内含子4a / b多态性的作用。

背景

一氧化氮（NO），氧化的低密度脂蛋白（OxLDL）和内皮型一氧化氮合酶内含子4a / b多态性（eNOSP）与动脉粥样硬化（AS）相关。本研究调查了常规有氧运动训练对上述危险因素以及血脂和脂蛋白（BLLP）的影响以及eNOSP的作用，目前尚不清楚。

方法

该研究由46名训练有素的男性足球裁判作为运动组（AG，年龄； 23.26±2.84岁）和43个久坐的对照组（CG，年龄； 23.16±3.28岁）参加。进行Yoyo间歇耐力（Yoyo IE-2测试）以测量参与者的有氧耐力水平。确定血清NO，eNOS和氧化LDL（OxLDL）水平（通过ELISA方法）以及总氧化剂/抗氧化剂状态比（/ TOS / TAS）作为氧化应激（OS）指数（OSI）和BLLPs水平。通过VNTR分析从基因组DNA样品中鉴定出eNOSP。

结果

AG和CG之间没有显着差异，包括NO，eNOS和BLLP的基因型组，eNOSP没有作用。但是，AG的NO（29％，p> .05）和TAS水平显着高于CG（p = .001），而OSI（p = .001）和OxLDL（p = .011）值显着低于CG 。另一方面，运动bb组的NO值较对照组和a载体（aC = aa + ab）组高29％。