By-products from biofuels production, such as crude glycerol and sugarcane molasses, are interesting carbon sources for anaerobic biological production of hydrogen and metabolites. The objective of this work was to promote the co-fermentation of those two substrates in an anaerobic fluidized bed reactor for obtaining biofuels and value-added products. The total and useful volumes of the reactor were 1.2 and 0.945 L, respectively. Sludge from a sewage treatment plant UASB reactor was used as the source of microorganisms. The crushed tire was applied as a support material to guarantee microbial adhesion. The molasses concentration was kept constant (4 g/L) and the crude glycerol varied (phase 1 = 1 g/L; phase 2 = 2 g/L, phase 3 = 3 g/L and phase 4 = 0 g/L) for a constant hydraulic retention time of 4 h. The highest average hydrogen yield (3.77 mol-H2/molglucose) and the highest average hydrogen production rate (0.34 L-H2/h Lreactor) occurred in the fourth phase of operation (without crude glycerol). The main soluble metabolites produced were 1,3-propanediol (14.4–38.8%), propionate (12.1–37.5%) and acetate (13.2–26.9%).

中文翻译：

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甘油和糖蜜的共发酵以获得生物燃料和增值产品

生物燃料生产的副产品，如粗甘油和甘蔗糖蜜，是厌氧生物生产氢气和代谢物的重要碳源。这项工作的目的是促进这两种底物在厌氧流化床反应器中的共发酵，以获得生物燃料和增值产品。反应器的总体积和有用体积分别为 1.2 和 0.945 L。来自污水处理厂 UASB 反应器的污泥用作微生物来源。压碎的轮胎用作支撑材料以保证微生物粘附。糖蜜浓度保持恒定 (4 g/L)，粗甘油变化（第 1 阶段 = 1 g/L；第 2 阶段 = 2 g/L，第 3 阶段 = 3 g/L 和第 4 阶段 = 0 g/L） 4 小时的恒定水力停留时间。最高的平均氢气产率（3. 77 mol-H2/molglucose）和最高的平均氢气产率（0.34 L-H2/h Lreactor）出现在第四个操作阶段（无粗甘油）。产生的主要可溶性代谢物是 1,3-丙二醇 (14.4-38.8%)、丙酸盐 (12.1-37.5%) 和乙酸盐 (13.2-26.9%)。