The role of lncRNA LEF1-AS1 in the regulation of osteogenic differentiation of dental pulp stem cells (DPSCs) is still obscure. Here, we demonstrated that LncRNA LEF1-AS1 expression was associated with osteogenic differentiation of DPSCs and overexpression of LEF1-AS1 promoted osteogenic differentiation. Moreover, lncRNA LEF1-AS1 and miR-24-3p could directly regulate each other and LEF1-AS1 acted as sponge partner of miR-24-3p. Furthermore, LEF1-AS1 and miR-24-3p synergized to regulate osteogenic differentiation of DPSCs. Finally, we verified TGFBR1 was the direct target of miR-24-3p in osteogenic differentiation of DPSCs and miR-24-3p/LEF1-AS1 sponged to regulate TGFBR1 expression. Our study revealed a novel mechanism about how did lncRNA LEF1-AS1 execute function in osteogenesis of DPSCs and thus might serve as potential therapeutic target for the bone regeneration in the dental pulp.

lncRNA LEF1-AS1在调节牙髓干细胞（DPSC）的成骨分化中的作用仍然不清楚。在这里，我们证明了LncRNA LEF1-AS1表达与DPSC的成骨分化有关，而LEF1-AS1的过表达促进了成骨分化。此外，lncRNA LEF1-AS1和miR-24-3p可以直接相互调节，而LEF1-AS1充当miR-24-3p的海绵伴侣。此外，LEF1-AS1和miR-24-3p协同调节DPSC的成骨分化。最后，我们证实TGFBR1是miR-24-3p在DPSC的成骨分化中的直接靶标，而miR-24-3p / LEF1-AS1可以调节TGFBR1的表达。