The objective of this study was to quantify the in vivo response of synoviocytes type A and B in the posterior joint capsule to knee immobilization and remobilization. Also, to correlate the immunohistochemical data with selected mRNA expression in the posterior joint capsule. Forty-two adult male Sprague–Dawley rats had one knee joint immobilized in flexion for durations of 1–4 weeks. Fifteen were harvested after immobilization and 15 were remobilized for 4 weeks. They were analyzed immunohistochemically with CD68 and CD55 antibodies as markers for synoviocytes type A and type B, respectively. Controls were 15 age-matched rats. The remaining 12 rats had their posterior capsule harvested and synoviocyte-specific CD68, CD55, and uridine diphosphoglucose dehydrogenase (UDPGD) mRNA expression was measured. Controls were 12 sham-operated knees. Knee immobilization for 2 weeks significantly increased synoviocytes A:B staining ratio compared to controls (3.88 ± 1.39 vs. 1.83 ± 0.76; p < 0.05). Remobilization for 4 weeks abolished the increase. Remobilization of knees that were immobilized for 1 week also significantly lowered the synoviocytes A:B staining ratios compared to immobilized-only knees (0.66 ± 0.23 vs. 2.19 ± 0.54; p < 0.05) and to controls (0.66 ± 0.23 vs. 1.32 ± 0.29; p < 0.05). Consistent with the immunohistochemistry, mRNA expression of synoviocyte type B-specific CD55 and UDPGD genes were significantly lower in the capsules immobilized for 2 weeks (both p < 0.05). Knee immobilization and remobilization significantly modulated synoviocytes in vivo, stressing their mechanosensitive nature and possible contribution to immobility-induced changes of the joint capsule.

这项研究的目的是量化后关节囊中的A型和B型滑膜细胞对膝关节固定和固定的体内反应。同样，将免疫组织化学数据与后关节囊中选定的mRNA表达相关。42只成年雄性Sprague-Dawley大鼠的一只膝关节屈曲固定，持续1-4周。固定后收获15只，固定15只，持续4周。用CD68和CD55抗体分别作为A型和B型滑膜细胞的标记进行了免疫组织化学分析。对照是15只年龄匹配的大鼠。其余12只大鼠的后囊被收获，并且滑膜细胞特异性CD68，CD55和尿苷二磷酸葡萄糖脱氢酶（UDPGD）mRNA表达被测量。对照是12只假手术的膝盖。与对照组相比，膝关节固定2周显着增加了滑膜细胞A：B染色率（3.88±1.39对1.83±0.76； p <0.05）。复员4周取消了增加。与仅固定膝盖相比（0.66±0.23 vs. 2.19±0.54; p <0.05）和对照组（0.66±0.23 vs 1.32± 0.29； p <0.05）。与免疫组织化学一致，滑膜细胞B型特异性CD55和UDPGD基因的mRNA表达在固定化2周的胶囊中显着降低（均p <0.05）。膝盖固定和固定在体内显着调节滑膜细胞，