Claudins are a family of integrated membrane-bound proteins involving in paracellular tightness, barrier forming, ion permeability, and substrate selection at tight junctions of chordate epithelial and endothelial cells. Here, 39 putative claudin genes were identified in the Pungitius sinensis based on the high throughput RNA-seq. Conservative motif distribution in each group suggested functional relevance. Divergence of duplicated genes implied the species’ adaptation to the environment. In addition, selective pressure analyses identified one site, which may accelerate functional divergence in this protein family. Pesticides cause environmental pollution and have a serious impact on aquatic organisms when entering the water. The expression pattern of most claudin genes was affected by organophosphorus pesticide, indicating that they may be involved in the immune regulation of organisms and the detoxification of xenobiotics. Protein–protein network analyses also exhibited 439 interactions, which implied the functional diversity. It will provide some references for the functional study on claudin genes.

中文翻译：

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Pungitius sinensis密蛋白基因的特征和分子进化

Claudins 是一个整合的膜结合蛋白家族，涉及细胞旁紧密性、屏障形成、离子渗透性和脊索动物上皮细胞和内皮细胞紧密连接处的底物选择。在这里，基于高通量 RNA-seq 在 Pungitius sinensis 中鉴定了 39 个假定的密蛋白基因。每组中的保守基序分布表明功能相关性。重复基因的差异意味着该物种对环境的适应。此外，选择性压力分析确定了一个位点，这可能会加速该蛋白质家族的功能分化。农药进入水中会造成环境污染，并对水生生物造成严重影响。大多数claudin基因的表达模式受有机磷农药的影响，表明它们可能参与生物体的免疫调节和异生物质的解毒。蛋白质-蛋白质网络分析还展示了 439 种相互作用，这意味着功能多样性。将为claudin基因的功能研究提供一些参考。