当前位置: X-MOL 学术Hum. Cell › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
miR-126a-3p targets HIF-1α and alleviates obstructive sleep apnea syndrome with hypertension.
Human Cell ( IF 3.4 ) Pub Date : 2020-08-10 , DOI: 10.1007/s13577-020-00404-z
Lirong He 1 , Xin Liao 1 , Guofeng Zhu 1 , Jiulong Kuang 1
Affiliation  

The obstructive sleep apnea syndrome (OSAS) is a common sleep-related breathing disorder and an important cause of refractory hypertension. MicroRNAs (miRNAs) are involved in the development of hypertension, but their role in OSAS with hypertension (OSAS-hypertension) has been little studied. Evidence indicates that miR-126a-3p expression is lower in patients with OSAS-hypertension compared with the patients with OSAS alone. However, its role in the pathogenesis of OSAS-hypertension remains unclear. Therefore, this study aims to investigate the role of miR-126a-3p in OSAS-hypertension and to determine whether HIF-1α is involved in this process. Sprague Dawley rats were exposed to chronic intermittent hypoxia (CIH) for 8 weeks to induce OSAS-hypertension. Rat aortic smooth muscle cells (A7r5) were cultured under hypoxia as an in vitro model. Our results showed that rats exposed to 8 week CIH exhibited decreased miR-126a-3p and increased HIF-1α expression. Furthermore, administration of recombinant adeno-associated virus expressing miR-126a-3p (rAAV-miR-126a) counteracted the CIH-induced systolic blood pressure upregulation, oxidase stress, inflammation, and heart and abdominal aorta vascular remodeling. Moreover, the mechanism was associated with its targeted suppression of HIF-1α. These findings suggest that miR-126a-3p might be a novel potential therapeutic target for the treatment of OSAS-hypertension.



中文翻译:

miR-126a-3p靶向HIF-1α并缓解高血压阻塞性睡眠呼吸暂停综合征。

阻塞性睡眠呼吸暂停综合症(OSAS)是一种常见的与睡眠有关的呼吸障碍,是顽固性高血压的重要原因。微小RNA(miRNA)参与高血压的发展,但其在高血压伴OSAS(OSAS-高血压)中的作用尚未得到研究。有证据表明,与单纯OSAS患者相比,OSAS高血压患者miR-126a-3p表达较低。但是,其在OSAS高血压发病机理中的作用仍不清楚。因此,本研究旨在调查miR-126a-3p在OSAS高血压中的作用,并确定HIF-1α是否参与该过程。将Sprague Dawley大鼠暴露于慢性间歇性缺氧(CIH)8周,以诱导OSAS高血压。在缺氧条件下培养大鼠主动脉平滑肌细胞(A7r5)作为体外模型。我们的结果表明,暴露于8周CIH的大鼠表现出减少的miR-126a-3p和增加的HIF-1α表达。此外,施用表达miR-126a-3p的重组腺相关病毒(rAAV-miR-126a)可以抵消CIH诱导的收缩压上调,氧化酶应激,炎症以及心脏和腹主动脉血管重塑。而且,该机制与其对HIF-1α的靶向抑制有关。这些发现表明,miR-126a-3p可能是治疗OSAS高血压的新型潜在治疗靶标。氧化酶应激,炎症以及心脏和腹主动脉血管重塑。而且,该机制与其对HIF-1α的靶向抑制有关。这些发现表明,miR-126a-3p可能是治疗OSAS高血压的新型潜在治疗靶标。氧化酶应激,炎症以及心脏和腹主动脉血管重塑。而且,该机制与其对HIF-1α的靶向抑制有关。这些发现表明,miR-126a-3p可能是治疗OSAS高血压的新型潜在治疗靶标。

更新日期:2020-08-11
down
wechat
bug