Sapajus nigritus are non-human primates which are widespread in South America. They are omnivores and live in troops of up to 40 individuals. The oral cavity is one of the main entry routes for microorganisms, including viruses. Our study proposed the identification of viral sequences from oral swabs collected in a group of capuchin monkeys (n = 5) living in a public park in a fragment of Mata Atlantica in South Brazil. Samples were submitted to nucleic acid extraction and enrichment, which was followed by the construction of libraries. After high-throughput sequencing and contig assembly, we used a pipeline to identify 11 viral families, which are Herpesviridae, Parvoviridae, Papillomaviridae, Polyomaviridae, Caulimoviridae, Iridoviridae, Astroviridae, Poxviridae, and Baculoviridae, in addition to two complete viral genomes of Anelloviridae and Genomoviridae. Some of these viruses were closely related to known viruses, while other fragments are more distantly related, with 50% of identity or less to the currently available virus sequences in databases. In addition to host-related viruses, insect and small vertebrate-related viruses were also found, as well as plant-related viruses, bringing insights about their diet. In conclusion, this viral metagenomic analysis reveals, for the first time, the profile of viruses in the oral cavity of wild, free ranging capuchin monkeys.

中文翻译：

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通过宏基因组分析从 Sapajus nigritus 口腔中的病毒多样性

Sapajus nigritus 是非人类灵长类动物，广泛分布于南美洲。它们是杂食性的，生活在多达 40 人的队伍中。口腔是微生物（包括病毒）的主要进入途径之一。我们的研究提议从生活在巴西南部马塔大西洋片段的公园中的一组卷尾猴（n = 5）收集的口腔拭子中鉴定病毒序列。样品进行核酸提取和富集，然后构建文库。经过高通量测序和重叠群组装，我们使用管道鉴定了 11 个病毒科，它们是疱疹病毒科、细小病毒科、乳头瘤病毒科、多瘤病毒科、花椰菜病毒科、虹彩病毒科、星状病毒科、痘病毒科和杆状病毒科，除了两个完整的病毒基因组，指环病毒科和基因病毒科。其中一些病毒与已知病毒密切相关，而其他片段则关系更远，与数据库中当前可用的病毒序列具有 50% 或更少的同一性。除了宿主相关病毒外，还发现了昆虫和小型脊椎动物相关病毒，以及植物相关病毒，从而了解它们的饮食。总之，这种病毒宏基因组分析首次揭示了野生卷尾猴口腔中病毒的特征。还发现了与昆虫和小型脊椎动物相关的病毒，以及与植物相关的病毒，从而深入了解了它们的饮食。总之，这种病毒宏基因组分析首次揭示了野生卷尾猴口腔中病毒的特征。还发现了与昆虫和小型脊椎动物相关的病毒，以及与植物相关的病毒，从而深入了解了它们的饮食。总之，这种病毒宏基因组分析首次揭示了野生卷尾猴口腔中病毒的特征。