In this study, we evaluated the saccharification potential of the secretome produced by a new elephant faeces isolate Aspergillus flavus (AF-NGF1), alone as well as in combination with a commercial enzyme (CTec2). Medium engineering (involving sequential Taguchi design, response surface methodology and one factor at a time approaches) enhanced the cellulase (FPase) secretion in the secretome of isolate AF-NGF1 (AF-S) by 3.89-folds. AF-S showed a maximum increase of 7.1- and 8.69-folds in exo-glucanase (avicellase) and endo-glucanase (CMCase) activities, respectively. Equal enzyme loading (20 FPU/g of biomass) of AF-S and CTec2 showed comparable saccharification potential with acid pre-treated paddy straw (APPS). However, a 1:1 combination of AF-S and CTec2 showed 67.84% and 37.21% increased saccharification of APPS as compared with AF-S or CTec2 alone at 50 °C and 40 °C, respectively. During simultaneous saccharification and fermentation (SSF) at 40 °C, a 1:1 combination produced > 2-folds increased ethanol titre as compared with AF-S or CTec2 alone. When equal FPU of AF-S and CTec2 were mixed for a total of 20 FPU, the degree of synergy (DOS) for APPS saccharification was 1.79 ± 0.06 and ~ 30.49% increased FPase activity was detected, which suggested the synergistic correlation between AF-S and CTec2. Therefore, the combination of AF-S and CTec2 could be considered as a potential cellulolytic enzyme formulation for efficient biomass hydrolysis and SSF process for lignocellulosic ethanol production.

在这项研究中，我们评估了由新的大象粪便分离物黄曲霉产生的分泌组的糖化潜力。（AF-NGF1），以及与市售酶（CTec2）组合使用。介质工程学（涉及顺序Taguchi设计，响应面方法和一次采用一个因素的方法）使分离的AF-NGF1（AF-S）分泌蛋白中的纤维素酶（FPase）分泌提高了3.89倍。AF-S分别显示外切葡聚糖酶（avicellase）和内切葡聚糖酶（CMCase）活性分别增加了7.1和8.69倍。AF-S和CTec2的等量酶负荷（20 FPU / g生物质）显示出与酸预处理稻草（APPS）相当的糖化潜力。但是，与单独的AF-S或CTec2在50°C和40°C相比，AF-S和CTec2的1：1组合显示APPS的糖化率分别提高了67.84％和37.21％。在40°C的同时糖化和发酵（SSF）期间，1：与单独的AF-S或CTec2相比，1种组合产生的乙醇滴度增加了2倍以上。当将相等FPU的AF-S和CTec2混合总共20 FPU时，APPS糖化的协同度（DOS）为1.79±0.06，并且检测到FPase活性增加了〜30.49％，这表明AF-S和CTec2之间的协同相关性S和CTec2。因此，AF-S和CTec2的组合可以被视为有效的生物质水解和SSF工艺生产木质纤维素乙醇的潜在纤维素分解酶制剂。这暗示了AF-S和CTec2之间的协同相关性。因此，AF-S和CTec2的组合可以被视为有效的生物质水解和SSF工艺生产木质纤维素乙醇的潜在纤维素分解酶制剂。这暗示了AF-S和CTec2之间的协同相关性。因此，AF-S和CTec2的组合可以被视为有效的生物质水解和SSF工艺生产木质纤维素乙醇的潜在纤维素分解酶制剂。