End-stage renal disease (ESRD) is a condition accompanied by increased inflammation, oxidative stress, risk of cardiovascular complications, and coagulopathies. The structure of fibrinogen and characteristics of fibrin from plasma samples of ESRD patients on peritoneal dialysis (PD) was investigated. Fibrinogen from ESRD patients had a higher degree of carbonylation than fibrinogen from healthy individuals. The Aα chain was the most susceptible to oxidation, followed by the Bβ chain, whereas the γ-chain was the most resistant to oxidation. Spectrofluorimetric analysis suggested a higher extent of modification of amino acid side chains in fibrinogen from ESRD patients. The tertiary structure of fibrinogen was more affected than its secondary structure. The kinetics (time and rate) of fibrinogen coagulation did not differ between the tested groups. Fibrin prepared from the isolated fibrinogen had a similar structure in both groups. Our results confirm that oxidation and structural alterations of fibrinogen occur in ESRD patients on PD, although these modifications produce no direct effect on the fibrin formation. Taking into account that some patients suffer from bleeding, whereas others develop thrombotic complications, further research on this subject is required to identify other components and processes that contribute to the outcome.

中文翻译：

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接受腹膜透析的终末期肾病患者的纤维蛋白原修饰和纤维蛋白形成

终末期肾病 (ESRD) 是一种伴随炎症、氧化应激、心血管并发症风险和凝血病增加的病症。研究了来自接受腹膜透析 (PD) 的 ESRD 患者血浆样品中纤维蛋白原的结构和纤维蛋白的特征。ESRD 患者的纤维蛋白原比健康个体的纤维蛋白原具有更高的羰基化程度。Aα 链最易氧化，其次是 Bβ 链，而 γ 链最耐氧化。荧光光谱分析表明 ESRD 患者纤维蛋白原中氨基酸侧链的修饰程度更高。纤维蛋白原的三级结构比其二级结构受到的影响更大。纤维蛋白原凝固的动力学（时间和速率）在测试组之间没有差异。从分离的纤维蛋白原制备的纤维蛋白在两组中具有相似的结构。我们的结果证实，PD 的 ESRD 患者发生纤维蛋白原的氧化和结构改变，尽管这些改变对纤维蛋白形成没有直接影响。考虑到一些患者会出现出血，而另一些患者会出现血栓并发症，因此需要对该主题进行进一步研究，以确定对结果有影响的其他成分和过程。