The full-length cDNA of cyclin C of the giant tiger shrimp Penaeus monodon (PmCyC) was isolated by RACE-PCR. It was 1443 bp in length containing an open reading frame (ORF) of 804 bp and 267 deduced amino acids. Tissue distribution analysis indicated that PmCyC was more abundantly expressed in ovaries and testes than other tissues of female and male juveniles (P < 0.05). A pair of primers was designed, and an amplification product of 403 bp containing an intron of 123 bp was obtained. Polymorphism of amplified PmCyC gene segments of the 5th (3-month-old G5, N = 30) and 7th (5-month-old G7, N = 18) generations of domesticated juveniles was analyzed. Four conserved SNPs (T>C₁₃₄, T>C₁₈₈, G>A₃₇₉, and T>C₃₈₂) were found within the examined sequences. A TaqMan genotyping assay was developed for detection of a T>C₁₃₄ SNP. Association analysis indicated that this SNP displayed significant association with body weight (P < 4.2e−10) and total length (P < 2e−09) of the examined G7 P. monodon (N = 419) with an allele substitution effect of 5.02 ± 0.78 g and 1.41 ± 0.19 cm, respectively. Juveniles with C/C₁₃₄ (22.80 ± 2.51 g and 12.97 ± 0.53 cm, N = 19) and T/C₁₃₄ (20.41 ± 0.93 g and 12.77 ± 0.21 cm, N = 129) genotypes exhibited a significantly greater average body weight and total length than those with a T/T₁₃₄ genotype (14.72 ± 0.53 g and 11.37 ± 0.13 cm, N = 271) (P < 0.05).

利用RACE-PCR技术，分离了大对虾斑节对虾（PmCyC）细胞周期蛋白C的全长cDNA 。它的长度为1443 bp，包含一个804 bp的开放阅读框（ORF）和267个推导的氨基酸。组织分布分析表明，PmCyC在卵巢和睾丸中的表达比雌，雄少年的其他组织都要丰富（P  <0.05）。设计一对引物，得到含有123bp内含子的403bp扩增产物。第五个（3个月大的G5，N  = 30）和第七个（五个月大的G7，N）扩增的PmCyC基因片段的多态性 = 18）分析了几代被驯化的少年。在检查的序列中发现了四个保守的SNP（T> C ₁₃₄，T> C ₁₈₈，G> A ₃₇₉和T> C ₃₈₂）。开发了TaqMan基因分型测定法，用于检测T> C ₁₃₄ SNP。关联分析表明，该SNP与 受检G7斑节对虾（N  = 419）的体重（P  <4.2e-10）和总长度（P <2e-09）显着相关，其等位基因替代效应为5.02±分别为0.78克和1.41±0.19厘米。C / C ₁₃₄（22.80±2.51 g和12.97±0.53 cm，N = 19）和T / C ₁₃₄（20.41±0.93 g和12.77±0.21 cm，N  = 129）基因型的平均体重和总长度显着大于T / T ₁₃₄基因型（14.72±0.53 g和11.37） ±0.13厘米，N  = 271）（P  <0.05）。