Background: Coxsackievirus infections are associated with cases of aseptic meningitis, encephalitis, myocarditis, and some chronic disease.

Methods: A series of benzo[d][1,2,3]triazol-1(2)-yl derivatives (here named benzotriazol-1(2)-yl) (4a-i, 5a-h, 6a-e, g, i, j and 7a-f, h-j) were designed, synthesized and in vitro evaluated for cytotoxicity and antiviral activity against two important human enteroviruses (HEVs) members of the Picornaviridae family [Coxsackievirus B 5 (CVB-5) and Poliovirus 1 (Sb-1)].

Results: Compounds 4c (CC50 >100 μM; EC50 = 9 μM), 5g (CC50 >100 μM; EC50 = 8 μM), and 6a (CC50 >100 μM; EC50 = 10 μM) were found active against CVB-5. With the aim of evaluating the selectivity of action of this class of compounds, a wide spectrum of RNA (positive- and negativesense), double-stranded (dsRNA) or DNA viruses were also assayed. For none of them, significant antiviral activity was determined.

Conclusion: These results point towards a selective activity against CVB-5, an important human pathogen that causes both acute and chronic diseases in infants, young children, and immunocompromised patients.

背景：柯萨奇病毒感染与无菌性脑膜炎，脑炎，心肌炎和某些慢性疾病有关。

方法：一系列苯并[d] [1,2,3]三唑-1（2）-基衍生物（此处称为苯并三唑-1（2）-基）（4a-i，5a-h，6a-e， g，i，j和7a-f，hj）被设计，合成并体外评估了对Picornaviridae家族的两个重要人类肠道病毒（HEV）成员[Coxsackievirus B 5（CVB-5）和脊髓灰质炎病毒1]的细胞毒性和抗病毒活性（Sb-1）]。

结果：发现化合物4c（CC50> 100μM； EC50 = 9μM），5g（CC50> 100μM； EC50 = 8μM）和6a（CC50> 100μM； EC50 = 10μM）具有抗CVB-5活性。为了评估这类化合物的作用选择性，还检测了广谱的RNA（正反义），双链（dsRNA）或DNA病毒。对于它们中没有一个，确定了显着的抗病毒活性。

结论：这些结果表明对CVB-5有选择性活性，CVB-5是一种重要的人类病原体，可引起婴儿，幼儿和免疫功能低下的患者的急性和慢性疾病。