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Comprehensive Investigation of Potential Novel Marine Psychrotolerant Actinomycetes sp. Isolated from the Bay-of-Bengal
Current Genomics ( IF 1.8 ) Pub Date : 2020-08-08 , DOI: 10.2174/1389202921666200330150642 Mrinmoy Ghosh 1 , Meeta Gera 2 , Joginder Singh 1 , Ram Prasad 3 , Krishna Kanth Pulicherla 4
Current Genomics ( IF 1.8 ) Pub Date : 2020-08-08 , DOI: 10.2174/1389202921666200330150642 Mrinmoy Ghosh 1 , Meeta Gera 2 , Joginder Singh 1 , Ram Prasad 3 , Krishna Kanth Pulicherla 4
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Background: This study was carried out to classify the diversity of the deep marine psychrotolerant actinomycetes sp. nov., in the Bay of Bengal and exploit the production of cold-active industrial and pharmaceutical biomolecules. Objective: 1) Characterization, optimum the growth conditions and classify the diversity of the novel isolated deep marine psychrotolerant actinomycetes sp from the Bay-of-Bengal. 2) Screening for industrially important biocatalysts and determine the antimicrobial activities against the five dreadful pathogens. 3) The differential expression profiling of the candidate genes to regulate the biosynthesis of selected enzymes. Methods: The cold-adapted actinomycetes were isolated from the deep marine water collections at 1200 mts below the surface in Bay-of-Bengal. The phenotypic and genotypic characterizations have been carried out to understand the persistent diversity of this novel marine psychrotolerant actinomycetes species. The production of cold-active enzymes, such as amylase, cellulase, lipase, pectinase, and L-asparaginase, were screened and the expression profiling genes were determined by using qRT PCR. The antibacterial and antifungal activities have also been investigated. Results: A total number of 37 novel actinomycetes were isolated and the phenotypic and genotypic characterizations identified the genus, dominated by Streptomyces (17 distinct sub-groups) as the major group, followed by Micromonospora, Actinopolyspora, Actinosynnema, Streptoverticillium, Saccharopolyspora, Nocardiopsis, and Nocardia. The optimum growth and abundant mycelium formation are observed at 15°C to 20°C and also capability for thriving at 4°C. All the isolates exhibited a significant role in the production of biocatalysts, and the antagonistic activities were also noted against five major selected pathogens. Conclusion: The Streptomyces from the Bay-of-Bengal have high biosynthetic potential and can serve as a good resource for the exploration of bioactive natural products.
中文翻译：
潜在新型海洋耐冷放线菌的综合研究。与孟加拉湾隔离
背景：本研究旨在对深海耐冷放线菌的多样性进行分类。11 月，在孟加拉湾开发冷活性工业和药物生物分子的生产。目的：1) 对来自孟加拉湾的新型分离的深海耐冷放线菌进行表征、优化生长条件并对其多样性进行分类。2) 筛选工业上重要的生物催化剂并确定对五种可怕病原体的抗菌活性。3) 候选基因的差异表达谱以调节所选酶的生物合成。方法：从孟加拉湾地表以下 1200 米的深海海水收集物中分离出适应冷的放线菌。已进行表型和基因型表征以了解这种新型海洋耐冷放线菌物种的持久多样性。筛选冷活性酶的产生，如淀粉酶、纤维素酶、脂肪酶、果胶酶和 L-天冬酰胺酶，并通过使用 qRT PCR 确定表达谱基因。还研究了抗菌和抗真菌活性。结果：共分离出 37 种新型放线菌，表型和基因型特征确定该属，以链霉菌属（17 个不同亚群）为主，其次是小单孢菌属、放线菌属、放线菌属、链轮孢菌属、糖多孢菌属、诺卡氏菌属和诺卡氏菌。在 15°C 至 20°C 下观察到最佳生长和丰富的菌丝体形成，并且在 4°C 下也能茁壮成长。所有分离株都在生物催化剂的生产中表现出重要作用，并且还注意到对五种主要选定病原体的拮抗活性。结论：来自孟加拉湾的链霉菌具有很高的生物合成潜力，可以作为探索具有生物活性的天然产物的良好资源。
更新日期：2020-08-08
中文翻译：
潜在新型海洋耐冷放线菌的综合研究。与孟加拉湾隔离
背景：本研究旨在对深海耐冷放线菌的多样性进行分类。11 月，在孟加拉湾开发冷活性工业和药物生物分子的生产。目的：1) 对来自孟加拉湾的新型分离的深海耐冷放线菌进行表征、优化生长条件并对其多样性进行分类。2) 筛选工业上重要的生物催化剂并确定对五种可怕病原体的抗菌活性。3) 候选基因的差异表达谱以调节所选酶的生物合成。方法：从孟加拉湾地表以下 1200 米的深海海水收集物中分离出适应冷的放线菌。已进行表型和基因型表征以了解这种新型海洋耐冷放线菌物种的持久多样性。筛选冷活性酶的产生，如淀粉酶、纤维素酶、脂肪酶、果胶酶和 L-天冬酰胺酶，并通过使用 qRT PCR 确定表达谱基因。还研究了抗菌和抗真菌活性。结果：共分离出 37 种新型放线菌，表型和基因型特征确定该属，以链霉菌属（17 个不同亚群）为主，其次是小单孢菌属、放线菌属、放线菌属、链轮孢菌属、糖多孢菌属、诺卡氏菌属和诺卡氏菌。在 15°C 至 20°C 下观察到最佳生长和丰富的菌丝体形成，并且在 4°C 下也能茁壮成长。所有分离株都在生物催化剂的生产中表现出重要作用，并且还注意到对五种主要选定病原体的拮抗活性。结论：来自孟加拉湾的链霉菌具有很高的生物合成潜力，可以作为探索具有生物活性的天然产物的良好资源。
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