With the ban of conventional cigarettes from public spaces, electronic cigarette (E-cig) liquids have emerged as a nicotine replacement treatment for smoking cessation. However, consumers possess little knowledge of the ingredients and health effects of E-cig liquids following exposure. This study evaluated hair cell damage and developmental toxicities following gestational exposure to E-cig liquids. Zebrafish embryos were exposed to E-cig liquids at different concentrations (0.1%, 0.2%, and 0.4%). Embryonic developmental toxicity and hair cell damage was evaluated at 6 and 7 d, respectively, after fertilization. The average number of hair cells in the anterior lateral line (ALL) and posterior lateral line (PLL) following E-cig exposure was compared to that of the control. Morphological abnormalities and heart rate were evaluated. E-cig liquids significantly damaged the hair cells in the ALL, compared to the control (control; 52.85 ± 5.29 cells, 0.1% E-cig; 49.43 ± 7.70 cells, 0.2% E-cig; 40.68 ± 12.00 cells, 0.4% E-cig; 32.14 ± 20.75%; n = 29–40; p < 0.01). At high concentrations, E-cig liquids significantly damaged the hair cells in the PLL (control; 36.88 ± 5.43 cells, 0.1% E-cig; 33.06 ± 5.21 cells, 0.2% E-cig; 30.95 ± 8.03 cells, 0.4% E-cig; 23.72 ± 15.53%, n = 29–40; p < 0.01). No morphological abnormalities in body shape, somites, notochord, tail, and pectoral fin were observed; however, abnormalities were observed in the dorsal fin and heart rate at high concentrations. Thus, gestational exposure to E-cigs significantly damaged hair cells in a concentration-dependent manner and induced developmental toxicities to the dorsal fin and heart rate at high concentrations.

随着公共场所禁止使用传统香烟，电子香烟 (E-cig) 液体已成为戒烟的尼古丁替代疗法。然而，消费者对接触电子烟液体的成分和健康影响知之甚少。这项研究评估了妊娠期接触电子烟液体后的毛细胞损伤和发育毒性。斑马鱼胚胎暴露于不同浓度（0.1%、0.2% 和 0.4%）的电子烟液体中。分别在受精后第 6 天和第 7 天评估胚胎发育毒性和毛细胞损伤。将电子烟暴露后前侧线 (ALL) 和后侧线 (PLL) 的平均毛细胞数与对照组进行比较。评估形态学异常和心率。与对照相比，电子烟液体显着损坏了 ALL 中的毛细胞（对照；52.85 ± 5.29 个细胞，0.1% 电子烟；49.43 ± 7.70 个细胞，0.2% 电子烟；40.68 ± 12.00 个细胞，0.4% E -cig；32.​​14 ± 20.75%；n = 29–40；p < 0.01）。在高浓度下，E-cig 液体显着损坏了 PLL 中的毛细胞（对照；36.88 ± 5.43 个细胞，0.1% E-cig；33.06 ± 5.21 个细胞，0.2% E-cig；30.95 ± 8.03 个细胞，0.4% E- cig；23.72 ± 15.53%，n = 29–40；p < 0.01）。体形、体节、脊索、尾、胸鳍未见形态异常；然而，在高浓度下观察到背鳍和心率异常。因此，