Aims. To evaluate the expression of DSG1 and DSG2 and investigate their clinicopathological significance in EHCC. Method. The protein expression of DSG1 and DSG2 was measured by EnVision immunohistochemistry in 15 normal biliary tract tissues, 10 biliary tract adenoma tissues, 30 peritumoral tissues, and 100 EHCC tumour tissues. Result. The expression of the DSG1 and DSG2 proteins was significantly lower in EHCC tumour tissues than in normal biliary tract tissues, biliary tract adenoma, and peritumoral tissues (). Adenoma and peritumoral tissues with negative DSG1 and/or DSG2 protein expression exhibited atypical hyperplasia. DSG1 expression was positively correlated with DSG2 expression in EHCC (). In patients with good differentiation, no invasion, no lymph metastasis, TNM I + II stage, and radical surgery, the positive expression of DSG1 and DSG2 proteins was higher (). In comparison to patients with negative DSG1 and/or DSG2 expression, the average overall survival time of those with positive expression was significantly longer (). Cox multivariate analysis revealed that negative DSG1 and DSG2 expressions were independent of poor prognosis factors in EHCC patients. The AUC calculated for DSG1 was 0.681 (95% confidence interval: 0.594–0.768) and that for DSG2 was 0.645 (95% confidence interval: 0.555–0.734), while that for DSG1 and DSG2 was 0.772 (95% confidence interval: 0.609-0.936). Conclusions. Negative protein expression of DSG1 and DSG2 is closely related to the pathogenesis, severe clinicopathological characteristics, aggressive biological behaviours, and dismal prognosis in EHCC.

中文翻译：

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作为预后生物标志物的 DSG1 和 DSG2 的负表达影响肝外胆管癌患者的总体生存率。

目标。评估DSG1和DSG2的表达并探讨其在EHCC中的临床病理学意义。方法。采用 EnVision 免疫组化方法检测了 15 个正常胆道组织、10 个胆道腺瘤组织、30 个瘤周组织和 100 个 EHCC 肿瘤组织中 DSG1 和 DSG2 的蛋白表达。结果。EHCC肿瘤组织中DSG1和DSG2蛋白的表达明显低于正常胆道组织、胆道腺瘤和瘤周组织。）。DSG1和/或DSG2蛋白表达阴性的腺瘤和瘤周组织表现出非典型增生。EHCC中DSG1的表达与DSG2的表达呈正相关（）。在分化良好、无浸润、无淋巴转移、TNMⅠ+Ⅱ期、根治性手术的患者中，DSG1、DSG2蛋白阳性表达较高（）。与DSG1和/或DSG2阴性表达的患者相比，阳性表达患者的平均总生存时间显着延长（）。Cox 多变量分析显示 DSG1 和 DSG2 的阴性表达与 EHCC 患者的不良预后因素无关。DSG1 计算的 AUC 为 0.681（95% 置信区间：0.594-0.768），DSG2 为 0.645（95% 置信区间：0.555-0.734），而 DSG1 和 DSG2 为 0.772（95% 置信区间：0.609- 0.936）。结论。DSG1和DSG2的阴性蛋白表达与EHCC的发病机制、严重的临床病理特征、侵袭性生物学行为和不良预后密切相关。