Biologically Functionalized Expanded Polytetrafluoroethylene Blood Vessel Grafts.,Biomacromolecules

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Biologically Functionalized Expanded Polytetrafluoroethylene Blood Vessel Grafts.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-08-10 , DOI: 10.1021/acs.biomac.0c00897
Dongfang Wang _{1,

2,

3,

4} , Yiyang Xu _{3,

4} , Yu-Jyun Lin _{3,

4} , Galip Yilmaz _{3,

4} , Jue Zhang ₅ , George Schmidt _{3,

4} , Qian Li _{1,

2} , James A Thomson ₅ , Lih-Sheng Turng _{3,

4}

Affiliation

Cardiovascular diseases plague human health because of the lack of transplantable small-diameter blood vessel (SDBV) grafts. Although expanded polytetrafluoroethylene (ePTFE) has the potential to be used as a biocompatible material for SDBV grafts, long-term patency is still the biggest challenge. As discussed in this paper, by virtue of a novel material formulation and a new and benign alcohol/water lubricating agent, biofunctionalized ePTFE blood vessel grafts aimed at providing long-term patency were fabricated. Compared to the most prevalent modification of PTFE, namely surface treatment, this method realized bulk treatment, which could guarantee homogeneous and long-lasting performance throughout PTFE products. These blood vessel grafts included embedded functional biomolecules, such as arginylglycylaspartic acid, heparin, and selenocystamine, using water as a solvent in paste extrusion and in the expansion of ePTFE. Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscope results confirmed the existence of these targeting biomolecules in the as-fabricated ePTFE blood vessel grafts. Meanwhile, the greatly improved biological functions of the grafts were demonstrated via live and dead assays, cell morphology, CD31 staining, nitric oxide (NO) release, and anticoagulation tests. This novel and benign material formulation and fabrication method provides an opportunity to produce multibiofunctional ePTFE blood vessel grafts in a single step, thus yielding a potent product with significant commercial and clinical potential.

中文翻译：

生物功能化的膨胀聚四氟乙烯血管移植物。

由于缺乏可移植的小直径血管（SDBV）移植物，心血管疾病困扰着人类健康。尽管膨体聚四氟乙烯（ePTFE）有潜力用作SDBV移植物的生物相容性材料，但长期开放仍然是最大的挑战。如本文所述，借助一种新颖的材料配方和一种新型且良性的酒精/水润滑剂，可以制造旨在提供长期开放性的生物功能化ePTFE血管移植物。与最流行的PTFE改性（即表面处理）相比，该方法实现了本体处理，可以保证整个PTFE产品的均质和持久性能。这些血管移植物包括嵌入式的功能性生物分子，例如精氨酰基糖基天冬氨酸，肝素和硒代半胱胺，在糊料挤出和ePTFE的膨胀中使用水作为溶剂。傅里叶变换红外光谱，X射线光电子能谱和扫描电子显微镜的结果证实了在制造的ePTFE血管移植物中存在这些靶向生物分子。同时，证明了移植物的生物学功能大大改善。通过活和死分析，细胞形态，CD31染色，一氧化氮（NO）释放和抗凝测试。这种新颖而良性的材料配方和制造方法为在单一步骤中生产多功能生物功能性ePTFE血管移植物提供了机会，从而产生了具有重大商业和临床潜力的有效产品。

更新日期：2020-09-14

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