Pantetheinase (Vanin-1) is an ectoenzyme, which involves the metabolic pathway of coenzyme A (CoA), and can decompose pantetheine into pantothenic acid (CoA precursor) and aminothiol cysteamine. Previous studies have revealed that Vanin-1 with essential biological functions is closely related to many diseases. However, the lack of simple and effective detection methods has severely hindered the further study of Vanin-1’s physiological functions. In this work, we have developed a near-infrared (NIR) emission ratio fluorescent probe TMN-PA (I_645 nm/I_568 nm) that enables us to detect Vanin-1 rapidly (in 15 min) with a minimum detection limit of 0.37 ng/mL. What is more, this probe shows excellent potential in in situ real-time monitoring of the endogenous Vanin-1, contributing to further research on Vanin-1 and understanding its mechanisms in physiological pathology. To our knowledge, this probe is the first NIR emission ratio (I_645 nm/I_568 nm) fluorescent probe ever reported to monitor the activity of Vanin-1 in vivo.

中文翻译：

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可见到近红外发射比率荧光探针，用于体内检测Vanin-1。

Pantetheinase（Vanin-1）是一种外切酶，涉及辅酶A（CoA）的代谢途径，可以将泛酸分解为泛酸（CoA前体）和氨基硫醇半胱胺。先前的研究表明，具有重要生物学功能的Vanin-1与许多疾病密切相关。然而，缺乏简单有效的检测方法严重阻碍了Vanin-1生理功能的进一步研究。在这项工作中，我们开发了一种近红外（NIR）发射比荧光探针TMN-PA（I _{645 nm} / I _{568 nm}），使我们能够在15分钟内快速检测Vanin-1，最低检测限为0.37 ng / mL。而且，该探针在内源性Vanin-1的原位实时监测中显示出极好的潜力，有助于进一步研究Vanin-1并了解其在生理病理学中的机制。据我们所知，该探针是有史以来第一个用于监测Vanin-1体内活性的NIR发射比（I _{645 nm} / I _{568 nm}）荧光探针。