SummaryExperiments were conducted to study in vitro maturation of prepubertal goat oocytes and their developmental potential after chemical activation. In Experiment 1, cumulus–oocytes complexes collected from the ovaries of prepubertal goats slaughtered at a local abattoir were matured in vitro in TCM-199-based medium supplemented with 10 µg/ml luteinizing hormone (LH) (treatment 1) or 10 µg/ml LH + 0.1 mM l-cysteine (treatment 2). In Experiment 2, mature oocytes were activated with either 5 µM ionomycin or 7% ethanol. After 18 h, some oocytes were randomly fixed and stained to evaluate their chromatin status, while others were cultured in embryo culture medium to study their further development. In Experiment 3, oocytes activated with 5 µM ionomycin were cultured for 7 days in one of the four different culture media [Charles Rosenkrans medium (CR-1), TCM-199, potassium simplex optimization medium (KSOM) and synthetic oviductal fluid (SOF)] to study their developmental potential. The maturation rate in control, treatment 1, and treatment 2 media did not differ from each other (P > 0.05). However, the lowest degeneration of oocytes was observed in treatment 3 (P < 0.05) when compared with the other two groups. The proportion of activated oocytes was higher, while non-activated oocytes were lower in ionomycin group when compared with the group activated with ethanol (P < 0.05). The proportions of oocytes cleaved were 65.7, 56.8, 61.0 and 54.4% in CR-1, TCM-199, KSOM and SOF medium, respectively, with no significant difference. However, further development of cleaved oocytes was better in KSOM followed by SOF.

中文翻译：

---

体外成熟和化学活化后青春期前山羊卵母细胞的发育

总结实验进行了研究体外青春期前山羊卵母细胞的成熟及其化学活化后的发育潜力。在实验 1 中，从当地屠宰场屠宰的青春期前山羊的卵巢中收集的卵丘-卵母细胞复合物成熟体外在补充有 10 µg/ml 黄体生成素 (LH)（处理 1）或 10 µg/ml LH + 0.1 mM 的 TCM-199 培养基中l-半胱氨酸（治疗2）。在实验 2 中，成熟的卵母细胞用 5 µM 离子霉素或 7% 乙醇激活。18小时后，一些卵母细胞被随机固定并染色以评估其染色质状态，而另一些则在胚胎培养基中培养以研究它们的进一步发育。在实验 3 中，用 5 µM 离子霉素激活的卵母细胞在四种不同培养基之一 [Charles Rosenkrans 培养基 (CR-1)、TCM-199、单纯钾优化培养基 (KSOM) 和合成输卵管液 (SOF) 中培养 7 天。 )] 研究他们的发展潜力。对照、处理 1 和处理 2 培养基中的成熟率彼此之间没有差异（磷> 0.05)。然而，在处理 3 中观察到卵母细胞的退化程度最低（磷< 0.05) 与其他两组相比。与乙醇活化组相比，离子霉素组活化卵母细胞比例较高，而未活化卵母细胞较低（磷< 0.05)。CR-1、TCM-199、KSOM和SOF培养基中卵母细胞裂解率分别为65.7%、56.8%、61.0%和54.4%，差异无统计学意义。然而，在 KSOM 中卵母细胞的进一步发育更好，其次是 SOF。