Previous studies have demonstrated that the independent stimulation of either electron transport or RuBP regeneration can increase the rate of photosynthetic carbon assimilation and plant biomass. In this paper, we present evidence that a multigene approach to simultaneously manipulate these two processes provides a further stimulation of photosynthesis. We report on the introduction of the cyanobacterial bifunctional enzyme fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase or the overexpression of the plant enzyme sedoheptulose-1,7-bisphosphatase, together with the expression of the red algal protein cytochrome c₆, and show that a further increase in biomass accumulation under both glasshouse and field conditions can be achieved. Furthermore, we provide evidence that the stimulation of both electron transport and RuBP regeneration can lead to enhanced intrinsic water-use efficiency under field conditions.

先前的研究表明，电子传递或RuBP再生的独立刺激可以增加光合碳同化率和植物生物量。在本文中，我们提供的证据表明，同时操纵这两个过程的多基因方法可以进一步刺激光合作用。我们报告了蓝藻双功能酶果糖-1,6-双磷酸酶/景天庚酮糖-1,7-双磷酸酶的引入或植物酶景天庚酮糖-1,7-双磷酸酶的过度表达，以及红藻蛋白细胞色素的表达c ₆ ，并表明在温室和田间条件下都可以实现生物量积累的进一步增加。此外，我们提供的证据表明，电子传输和 RuBP 再生的刺激可以导致现场条件下内在水分利用效率的提高。